Diagnostic capabilities of ejaculate sediment examination with the help of nucleic acid amplification by polymerase chain reaction and microbiologic examination in searching for causes of male reproductive disfunction

Sapozhkova Zh.Yu.

International Cytology School and Medical School of Innovations;
Podolsk Diagnostic Center LLC

Milovanova G.A.

Podolsk Diagnostic Center LLC;
Russian Medical Academy of Postgraduate Professional Education

Pochernikov D.G.

Ivanovo State Medical Academy

Postovoitenko N.T.

Mother and Child Vladimir LLC

Diagnostic capabilities of ejaculate sediment examination with the help of nucleic acid amplification by polymerase chain reaction and microbiologic examination in searching for causes of male reproductive disfunction

Authors:

Sapozhkova Zh.Yu., Milovanova G.A., Pochernikov D.G., Postovoitenko N.T.

More about the authors

Journal: Medical Technologies. Assessment and Choice. 2022;44(2): 62‑71

DOI: 10.17116/medtech20224402162

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To cite this article:

Sapozhkova ZhYu, Milovanova GA, Pochernikov DG, Postovoitenko NT. Diagnostic capabilities of ejaculate sediment examination with the help of nucleic acid amplification by polymerase chain reaction and microbiologic examination in searching for causes of male reproductive disfunction. Medical Technologies. Assessment and Choice. 2022;44(2):62‑71. (In Russ.)
https://doi.org/10.17116/medtech20224402162

Подписка 2025 (Medical Technologies. Assessment and Choice)

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OBJECTIVE

To analyze the diagnostic capabilities of nucleic acid amplification (NAA) by real-time polymerase chain reaction (PCR) and microbiological examination of ejaculate sediment in determining the causes of male reproductive dysfunction.

MATERIAL AND METHODS

We examined 529 samples of ejaculate sediment from men aged 18—59 years with infertility and male accessory gland infection (Male Accessory Gland Infection/MAGI) between 2017 and 2021. Microorganisms for microbiological and molecular analysis were identified by matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS) and real-time PCR (Androflor test, NAAT/PCR-RV/Androflor).

RESULTS

As a rule, significant bacteriospermia in ejaculate sediment was detected in Androflor molecular test compared to microbiological examination (402 (76%) and 237 (44.8%), respectively, p<0.01). Analysis of biotope by two methods indicates complete coincidence of microbial status only in 40 (7.6%) out of 529 cases. Otherwise, microbiological examination found no microbiota (83 (15.7%) sample) or spectrum of detected microorganisms differed. There were differences in the structure of isolated groups of microorganisms in cluster of predominant obligate anaerobic conditionally pathogenic microorganisms (p<0.01), gram-positive facultative anaerobic microorganisms Pseudomonas aeruginosa/Ralstonia spp./Burkholderia spp. (p<0.01), Haemophilus spp. (p<0.01) and monoculture Enterobacteriaceae spp./Enterococcus spp. (p<0.01). In the group with normal flora, NAA/RT-PCR/Androflor test revealed common associations of microorganisms (103 (81.1%)), microbiological examination revealed monoculture in most cases (216 (74%)) (p<0.01).

CONCLUSION

The Androflor molecular test is recommended for effective laboratory examination and timely treatment of men with reproductive dysfunction. Microbiological examination is also important since this method is valuable to analyze sensitivity of microorganisms to antibiotics and detect various enterobacteria requiring different therapeutic regimens.

Keywords:

ejaculate sediment analysis

RT-PCR

Androflor

microbiological analysis

male accessory gland infection

male infertility

Authors:

Sapozhkova Zh.Yu.

International Cytology School and Medical School of Innovations;
Podolsk Diagnostic Center LLC

Milovanova G.A.

Podolsk Diagnostic Center LLC;
Russian Medical Academy of Postgraduate Professional Education

Pochernikov D.G.

Ivanovo State Medical Academy

Postovoitenko N.T.

Mother and Child Vladimir LLC

Received:

21.02.2022

Accepted:

25.03.2022

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