Relevance. Among the new drugs with a prolonged effect of biorevitalization, a complex of hyaluronic acid with trehalose (HAT) is attracting attention. The advantage of the complex is the ability of trehalose to maintain the vital activity of cells in adverse environmental conditions. The purpose of the study was investigating the duration of the clinical effect, as well as the dynamics of the functional parameters of the skin after a course of mesotherapy with a drug containing a complex HAT, women with external signs of aging. Material and methods. The study included 20 women (average age 45.1±7.3 years) with external signs of aging, who underwent 2 mesotherapy procedures with a HAT complex in the skin of the face and neck with an interval of 1 month. The examination included questioning of patients, photographing, assessment of the functional parameters of the skin using the methods of corneometry, mexametry, ultrasonography of the skin, followed by photographing the images obtained, as well as measuring its elasticity. An instrumental study was carried out before the start of treatment, a week after the course of procedures, and also 3 and 6 months after the first procedure. Results. It was determined that the use of a drug containing the HAT complex had a positive effect on the clinical signs of aging, reduced the degree of skin pigmentation and erythema, increased its elasticity, and also led to an increase in the density of the dermis according to ultrasound data. The indicated effect persisted for 6 months of observation. Conclusion. A drug containing a HAT complex has a pronounced effect on the manifestations of skin aging, is well tolerated by patients and is stored in the skin for at least six months.