Spectrophotometric intradermal analysis is a modern non-invasive examination method for the outer skin layers. Experienced specialist is required for successful diagnosis using sophisticated equipment. Objective — the study was aimed at determining the diagnostic value of the spectrophotometric intradermal diagnosis of melanocytic skin tumors, depending on the experience of a dermatologist. Material and methods. The study was carried out based on SIAsсans consisting of 200 image complexes. When comparing diagnostic value of SIAscan analysis of melanocytic neoplasms by dermatologists with experience of more than 5 years and less than 3 years, sensitivity of melanoma diagnosis was 71.8% in the case of less experienced dermatologist, which was significantly lower (p<0.001), than in the case of the dermatologist with long work experience (95.2%). There was no significant difference in the specificity of melanoma diagnosis. Significant difference (p<0.001) in diagnostic accuracy of SIAscan analysis by inexperienced and experienced dermatologists was found for skin melanoma, which amounted to 95.4 and 85.1%, respectively, due to the low sensitivity. Inclusion of the information about non-invasive methods of skin tumors diagnosis in the educational programs, cycles of training for dermatologists, cosmetologists, and oncologists will improve the capabilities of specialists in difficult diagnostic cases.