Regulatory miRNA21, miRNA92, miRNA221 and miRNAlet7 expression in carotid and coronary atherosclerotic plaques

Soboleva TV, Shchekochikhin DYu, Demura TA, et al. . Regulatory miRNA21, miRNA92, miRNA221 and miRNAlet7 expression in carotid and coronary atherosclerotic plaques. Russian Journal of Cardiology and Cardiovascular Surgery. 2024;17(3):245‑252. (In Russ.)
https://doi.org/10.17116/kardio202417031245

Подписка 2026 Кардиология и сердечно-сосудистая хирургия (Russian Journal of Cardiology and Cardiovascular Surgery)

Использование инфографики авторами научных работ

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OBJECTIVE

To analyze expression of microRNAs miRNAlet7a, miRNA21, miRNA92 and miRNA221 in reversible atherosclerotic plaques.

MATERIAL AND METHODS

The main group included patients with coronary endarterectomy during coronary artery bypass grafting (n=6) and patients after carotid endarterectomy (n=5). The control group included specimens of coronary arteries in 6 patients with atherosclerosis who died from non-cardiac causes. Molecular genetic analysis of microRNA was carried out using in situ hybridization with LNA probes. We analyzed expression of short microRNAs in carotid and coronary plaques, as well as relationship between microRNA and stability/instability of atherosclerotic plaques. The Mann-Whitney U test was used to compare microRNA expression in coronary and carotid arteries.

RESULTS

According to the Mann-Whitney test for expression of microRNA 221 in smooth muscle cells, the apparent value was greater than critical value at initialization level of <0.05. This demonstrates a significant relationship between microRNA 221 expression in smooth muscle cells and plaque localization in coronary arteries. There was significantly more active microRNA 221 staining in smooth muscle cells of coronary artery plaques compared to carotid arteries (p<0.05).

CONCLUSION

Increased expression of some microRNAs was revealed in unstable atherosclerotic plaques in coronary and carotid atherosclerosis. However, expression of microRNA 221 is characteristic exclusively for coronary lesions.

Keywords:

expression

microRNA

coronary atherosclerosis

carotid artery lesion

Authors:

Soboleva T.V.

Sechenov First Moscow State Medical University

ORCID: 0000-0003-4509-1068

Shchekochikhin D.Yu.

Sechenov First Moscow State Medical University

ORCID: 0000-0002-8209-2791

Demura T.A.

Sechenov First Moscow State Medical University

SPIN RSCI: 2198-5765
Scopus AuthorID: 25936132400
ORCID: 0000-0002-6946-6146

Babayan G.K.

Sechenov First Moscow State Medical University

ORCID: 0000-0001-5109-7795

Stonogina D.A.

Sechenov First Moscow State Medical University

ORCID: 0000-0002-1508-4257

Vasiliev S.V.

Sechenov First Moscow State Medical University

ORCID: 0000-0003-4845-5402

Lomonosova A.A.

Sechenov First Moscow State Medical University

ORCID: 0000-0002-2875-6155

Zakharevich V.M.

Sechenov First Moscow State Medical University

ORCID: 0000-0002-1090-6901

Shevchenko O.P.

Sechenov First Moscow State Medical University

ORCID: 0000-0001-6661-146X

Received:

15.09.2023

Accepted:

18.10.2023

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