Tumor necrosis factor-alpha as a modulator of secretory activity and protein cargo of extracellular vesicles in mesenchymal stromal cells

Zubkova ES, Beloglazova IB, Kalinin AP, Ratner EI, Men’shikov MYu, Parfyonova YeV, Tsokolaeva ZI. Tumor necrosis factor-alpha as a modulator of secretory activity and protein cargo of extracellular vesicles in mesenchymal stromal cells. Russian Cardiology Bulletin. 2024;19(2):13‑20. (In Russ.)
https://doi.org/10.17116/Cardiobulletin20241902113

Подписка 2026 Кардиологический вестник (Russian Cardiology Bulletin)

Использование инфографики авторами научных работ

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Abstract:

Mesenchymal stem cells (MSCs) are essential in regulating immune response and tissue recovery after inflammation. They are capable to change own secretome in inflammatory conditions. We investigated the influence of tumor necrosis factor-alpha (TNF-alpha) on composition of MSC secretome in soluble fraction and extracellular vesicles. We found significantly higher secretion of cytokines (lymphotoxin alpha, IFN-gamma and IL-7) contributing to organization of tertiary lymphoid structures. There were changes in protein content of extracellular vesicles, primarily in proteins involved in cytoskeleton, adhesion and chemotaxis. Increased levels of chaperones and proteins associated with response to oxidative stress in extracellular vesicles may provide transmission of anti-stress protective factors. This fact further emphasizes significance of MSCs in immune regulation.

Keywords:

mesenchymal stromal cells

tumor necrosis factor

secretome

extracellular vesicles

proteome

Authors:

Zubkova E.S.

Chazov National Medical Research Center of Cardiology

ORCID: 0000-0002-0512-3670

Beloglazova I.B.

Chazov National Medical Research Center of Cardiology

ORCID: 0000-0002-2345-3681

Kalinin A.P.

Chazov National Medical Research Center of Cardiology;
Lomonosov Moscow State University

ORCID: 0000-0002-9668-3832

Ratner E.I.

Chazov National Medical Research Center of Cardiology

ORCID: 0000-0003-0920-4029

Men’shikov M.Yu.

Chazov National Medical Research Center of Cardiology

ORCID: 0000-0002-9949-0534

Parfyonova Ye.V.

Chazov National Medical Research Center of Cardiology;
Lomonosov Moscow State University

ORCID: 0000-0002-0969-5780

Tsokolaeva Z.I.

Chazov National Medical Research Center of Cardiology;
Federal Research Clinical Center of Intensive Care and Rehabilitology

ORCID: 0000-0003-2441-6062

Received:

23.11.2023

Accepted:

26.12.2023

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References:

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