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Ivina A.A.

Kafedra patologicheskoĭ anatomii meditsinskogo fakul'teta FGBOU VPO "Rossiĭskiĭ universitet druzhby narodov"

Semkin V.A.

Otdelenie khirurgicheskoĭ stomatologii FGBU "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut stomatologii i cheliustno-litsevoĭ khirurgii" Minzdrava RF, Moskva

Khabadze Z.S.

People’s Friendship University of Russia, Moscow, Russia

Babichenko I.I.

Russian University of People’s Friendship, Moscow, Russia, 117198

Immunohistochemical study of Ki-67, PHH3, and CK15 protein expression in oral epithelial malignancy

Authors:

Ivina A.A., Semkin V.A., Khabadze Z.S., Babichenko I.I.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2019;81(5): 30‑34

DOI: 10.17116/patol20198105130

Views: 2575

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To cite this article:

Ivina AA, Semkin VA, Khabadze ZS, Babichenko II. Immunohistochemical study of Ki-67, PHH3, and CK15 protein expression in oral epithelial malignancy. Russian Journal of Archive of Pathology. 2019;81(5):30‑34. (In Russ., In Engl.)
https://doi.org/10.17116/patol20198105130

Подписка 2025 (Russian Journal of Archive of Pathology)
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Objective — to investigate the expression of Ki-67, phosphohistone H-3 (PHH3), and cytokeratin 15 (CK15) proteins in the cells of the oral mucosa (OM) according to the degree of its malignant transformation. Material and methods. OM biopsy specimens from 69 patients diagnosed with focal epithelial hyperplasia, intraepithelial squamous cell neoplasia, cancer in situ, and squamous cell carcinoma were examined. Tissue antigens were determined using mouse Ki-67 monoclonal antibodies, rabbit PHH3 polyclonal antibodies, and mouse CK15 monoclonal antibodies. Results. There was an increase in epithelial proliferative and mitotic activities in squamous cell carcinoma and a sharp decrease in the expression of CK15 in the cytoplasm in cancer in situ and squamous cell carcinoma of the OM. Conclusion. The protein CK15 can be used for the differential diagnosis between high-grade dysplasia and OM epithelial malignancy at the stage of carcinoma in situ and squamous cell carcinoma.

Keywords:

immunohistochemical study
squamous cell carcinoma
Ki-67
PHH3
CK15

Authors:

Ivina A.A.

Kafedra patologicheskoĭ anatomii meditsinskogo fakul'teta FGBOU VPO "Rossiĭskiĭ universitet druzhby narodov"

Semkin V.A.

Otdelenie khirurgicheskoĭ stomatologii FGBU "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut stomatologii i cheliustno-litsevoĭ khirurgii" Minzdrava RF, Moskva

Khabadze Z.S.

People’s Friendship University of Russia, Moscow, Russia

Babichenko I.I.

Russian University of People’s Friendship, Moscow, Russia, 117198

List of references:

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  2. Barakat SM, Siar CH. Differential expression of stem cell-like proteins in normal, hyperplastic and dysplastic oral epithelium. J Appl Oral Sci. 2015;23(1):79-86. https://doi.org/10.1590/1678-775720140245
  3. Mashhadiabbas F, Fayazi-Boroujeni M. Correlation of vascularization and inflammation with severity of oral leukoplakia. Iran J Pathol. 2017;12(3):225-230.
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  7. Ivina AA, Semkin VA, Babichenko II. Cytokeratin 15 as a diagnostic marker for oral epithelial malignization. Stomatologiya. 2018;97(6):61-62. (In Russ.) https://doi.org/10.17116/stomat20189706161
  8. Leonardi E, Girlando S, Serio G, Mauri FA, Perrone G, Scampini S, Dalla Palma P, Barbareschi M. PCNA and Ki-67 expression in breast carcinoma: correlation with clinical and biological variables. J Clin Pathol. 1992;45(5):416-419. https://doi.org/10.1136/jcp.45.5.416
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  10. Bose A, Teh MT, Hutchison IL, Wan H, Leigh IM, Waseem A. Two mechanisms regulate keratin K15 expression in keratinocytes: role of PKC/AP-1 and FOXM1 mediated signalling. PLoS One. 2012;7(6):e38599. https://doi.org/10.1371/journal.pone.0038599
  11. Ivina AA, Semkin VA, Babichenko II. Morphology of epithelial-mesenchymal transformation in neoplasias of oral mucosa. Stomatologiya. 2017;96(5):11-13. (In Russ.) https://doi.org/10.17116/stomat201796511-13
  12. Seltmann K, Roth W, Kröger C, Loschke F, Lederer M, Hüttelmaier S, Magin TM. Keratins mediate localization of hemidesmosomes and repress cell motility. J Invest Dermatol. 2013;133(1):181-190. https://doi.org/10.1038/jid.2012.256
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