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Nikitin P.V.

Burdenko Neurosurgery Institute, 4-ya Tverskaya-Yamskaya Str., 16, Moscow, Russia, 125047

Ryzhova M.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Zubova I.V.

Acad. N.N. Burdenko National Medical Research Center of Neurosurgery, Ministry of Health of Russia, Moscow, Russia

Panina T.N.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Shugay S.V.

Burdenko Neurosurgical Institute, Moscow, Russia, 125047

Heterogeneity of tumor cells in glioblastomas (in Russian only)

Authors:

Nikitin P.V., Ryzhova M.V., Zubova I.V., Panina T.N., Shugay S.V.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2019;81(3): 27‑36

DOI: 10.17116/patol20198103127

Read: 2975 times

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Table of contents

HETEROGENEITY OF TUMOR CELLS IN GLIOBLASTOMAS (IN RUSSIAN ONLY)
HETEROGENEITY OF TUMOR CELLS IN GLIOBLASTOMAS (IN RUSSIAN ONLY)

To cite this article:

Nikitin PV, Ryzhova MV, Zubova IV, Panina TN, Shugay SV. Heterogeneity of tumor cells in glioblastomas (in Russian only). Russian Journal of Archive of Pathology. 2019;81(3):27‑36. (In Russ.)
https://doi.org/10.17116/patol20198103127

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Objective — to comprehensively assess the functional molecular biological status of different tumor cell populations in glioblastoma samples. Material and methods. The activity of Ki-67, Bcl-2, and BCL6 expression was determined in 20 tumor samples from patients with glioblastoma. After that, a spatial analysis of heterogeneity in the expression of these markers in different tumor cell populations was carried out using computer and software tools and calculating the percentage of cells (PC) expressing this marker (Ki-67 labeling index (LI)) and a modified histoscore in different cell clusters. Results. Analysis of heterogeneity in the distribution of Ki-67, Bcl-2, and BCL6 expression could identify five cell clusters differing in the expression level of the above-mentioned markers. The most active cluster was the perivascular one (the highest mean Ki-67 LI (22.23±1.4%) and histoscore (118.59±3.36%); BCL6 PC and histoscore (17.4±1.4 and 79.32±4.86%, respectively). The least proliferative activity was observed in the perinecrotic cluster (Ki-67 LI (6.83±0.5%) and histoscore (62.46±2.25%)), while the neighboring transient necrotic cluster displayed sufficiently active proliferative processes (Ki-67 LI (18.39±0.56%) and histoscore (112.65±2.76%)). In addition, the transient vascular cluster was noted for a low proliferative activity (Ki-67 LI (8.37±0.35%) and histoscore (75.48±2.04%)). Finally, the intermediate cluster was characterized by the mean values of all parameters (Ki-67 LI (10.68±0.39%) and histoscore (95.73±2.37%). It should be noted that the differences in the expression activity for markers in these clusters were statistically significant. Conclusion. The perivascular cluster carries the greatest potential for tumor progression and recurrence, which agrees with the data available in the literature: the perivascular zone is the most important niche for glioma stem cells that make a considerable contribution to the malignant potential of glioblastoma. Tumor pathogenesis and morphogenesis are a complex interweaving of interrelated factors, the realization of which within the framework of a multi-level heterochronic pathological process leads to the segregation of tumor cells and to the appearance of separate cell populations described in this paper.

Keywords:

glioblastoma
cell clusters
functional cell activity
personalized medicine

Authors:

Nikitin P.V.

Burdenko Neurosurgery Institute, 4-ya Tverskaya-Yamskaya Str., 16, Moscow, Russia, 125047

Ryzhova M.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Zubova I.V.

Acad. N.N. Burdenko National Medical Research Center of Neurosurgery, Ministry of Health of Russia, Moscow, Russia

Panina T.N.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Shugay S.V.

Burdenko Neurosurgical Institute, Moscow, Russia, 125047

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