OBJECTIVE

To evaluate the toxic effects of the agent Relatox on mature outbred rats and mice in an acute experiment in comparison with the registered analogue Dysport.

MATERIAL AND METHODS

Based on the aim of experiment, the acute toxic effects of Relatox and Dysport were assessed on two animal species: rats and mice at intraperitoneal and intramuscular administration at dose levels that made it possible to calculate the toxicological parameter values (initially 10—150 U/kg with subsequent usage of additional doses 20 U/kg to 300 U/kg depending on the agent and route of administration). The LD₅₀ values and other acute toxic parameters were calculated using probit analysis.

RESULTS

The manifested intoxication picture was observed following administration of both agents, in which neurological symptoms specific to botulinum toxin came to the fore: muscle paresis and local paralysis, which were the result of the mode of therapeutic action of the toxin. The observed picture of intoxication, monitoring of the dynamics of body weight gains, as well as data of macroscopic analysis of organs and tissues indicate a complete analogy of animal responses to both agents administration. However, the mortality rates of animals differed when administered at different doses as well as the established LD₅₀ values of Relatox and Dysport.

CONCLUSIONS

Both agents were more toxic when administered intraperitoneally than intramuscularly. Species or gender sensitivities were no detected for both agents. LD₅₀ values of Relatox and Dysport established based on the results of an acute experiment, should be considered taking into account the differences (approximately 2.5—3-folds) in the specific activity of these agents.