The effect of natural mineral bicarbonate sodium-calcium water «Tib-1» on the antioxidant status of rats with adjuvant rheumatoid arthritis

Skupnevskii S.V.; Batagova F.E.; Pukhaeva E.G.; Badtiev A.K.; Rurua F.K.; Farnieva Zh.G.

doi:https://doi.org/10.17116/kurort20229903141

Skupnevskii S.V.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Batagova F.E.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Pukhaeva E.G.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Badtiev A.K.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Rurua F.K.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Farnieva Zh.G.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

The effect of natural mineral bicarbonate sodium-calcium water «Tib-1» on the antioxidant status of rats with adjuvant rheumatoid arthritis

Authors:

Skupnevskii S.V., Batagova F.E., Pukhaeva E.G., Badtiev A.K., Rurua F.K., Farnieva Zh.G.

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Journal: Problems of Balneology, Physiotherapy and Exercise Therapy. 2022;99(3): 41‑49

DOI: 10.17116/kurort20229903141

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Skupnevskii SV, Batagova FE, Pukhaeva EG, Badtiev AK, Rurua FK, Farnieva ZhG. The effect of natural mineral bicarbonate sodium-calcium water «Tib-1» on the antioxidant status of rats with adjuvant rheumatoid arthritis. Problems of Balneology, Physiotherapy and Exercise Therapy. 2022;99(3):41‑49. (In Russ.)
https://doi.org/10.17116/kurort20229903141

Подписка 2026 Вопросы курортологии, физиотерапии и лечебной физической культуры (Problems of Balneology, Physiotherapy and Exercise Therapy)

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PURPOSE OF THE STUDY

Study of the effect of natural mineral water « Tib-1» on the lipid peroxidation system in an experiment with a model adjuvant-induced RA in rats.

MATERIAL AND METHODS

The object of the study were Wistar rats, divided into three groups: negative control (solvents), positive control (model of adjuvant-induced RA by subcutaneous injection of complete Freund’s adjuvant) and experimental (correction of RA with mineral water « Tib-1», diluted in a ratio of 1:3 during the first 2 weeks from the moment the model was formed in the ad libitum mode). On the 3rd and 7th weeks in the blood of the animals were determined: the total number of leukocytes, the content of hydroperoxides according to Gavrilov, the level of malondialdehyde (MDA), catalase activity. Pathological changes in the hip and knee joints were recorded using radiography.

RESULTS

The inflammatory process in the positive control group by the 3rd week was characterized by an increase in the number of leukocytes by 66% (p<0.01) and was accompanied by an increase in MDA by 60% (p<0.001). By the 7th week, despite a relative increase in catalase activity (16%), the MDA level continued to be elevated compared to the negative control by 67% (p<0.001). Against the background of exposure to mineral water, inflammation decreased (the number of leukocytes in the “model/experiment” groups turned out to be reduced by 41%; p<0.01) and an increase in compensatory-adaptive reactions in the form of catalase activation was noted (by 8%; p<0.01), which was accompanied by a persistent (weeks 3 and 7) decrease in MDA output (by 20%; p<0.01). Using the method of radiation diagnostics, positive changes in the articular apparatus of experimental animals were revealed, consisting in the relief of signs of subchondral sclerosis of the bone heads, which were noted for animals of the model group.

CONCLUSION

The use of natural mineral water «Tib-1» helps to reduce the acute inflammatory response during the formation of adjuvant-induced RA in Wistar rats, initiates the normalization of the balance of pro- and antioxidant processes in the body, and minimizes the intensity of degenerative-inflammatory joint lesions.

Keywords:

autoimmune pathologies

balneotherapy

cartilage degenerative changes

mineral water

rheumatoid arthritis

synovial membrane

connective tissue

Authors:

Skupnevskii S.V.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Batagova F.E.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Pukhaeva E.G.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Badtiev A.K.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Rurua F.K.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Farnieva Zh.G.

Institute of Biomedical Investigations — the Affiliate of Vladikavkaz Scientific Centre of RAS

Received:

24.09.2021

Accepted:

26.10.2021

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