Assessment of the phenotypic composition of lymphocytes, immunoglobulins and their relationships in patients with chronic rinosinusitis

Smirnova O.V.; Sinyakov A.A.; Markina A.N.; Parilova O.V.

doi:https://doi.org/10.17116/otorino20238806142

Smirnova O.V.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0003-3992-9207

Sinyakov A.A.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0002-4474-1893

Markina A.N.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0001-9539-4417

Parilova O.V.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0003-2948-9658

Assessment of the phenotypic composition of lymphocytes, immunoglobulins and their relationships in patients with chronic rinosinusitis

Authors:

Smirnova O.V., Sinyakov A.A., Markina A.N., Parilova O.V.

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Journal: Russian Bulletin of Otorhinolaryngology. 2023;88(6): 42‑47

DOI: 10.17116/otorino20238806142

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To cite this article:

Smirnova OV, Sinyakov AA, Markina AN, Parilova OV. Assessment of the phenotypic composition of lymphocytes, immunoglobulins and their relationships in patients with chronic rinosinusitis. Russian Bulletin of Otorhinolaryngology. 2023;88(6):42‑47. (In Russ.)
https://doi.org/10.17116/otorino20238806142

Подписка 2026 Вестник оториноларингологии (Russian Bulletin of Otorhinolaryngology)

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OBJECTIVE

The study of the population and subpopulation content of lymphocytes and immunoglobulins and their associations in IgE-mediated CRS relative to other CRS and the control group.

MATERIAL AND METHODS

23 patients with IgE-mediated chronic rhinusinusitis and 67 patients with normal IgE blood levels were examined. For analysis, flow cytometry (Cytomics FC500, Beckman Coulter, USA), using monoclonal antibodies CD3⁺, CD4⁺, CD8⁺, CD16⁺, CD19⁺ and enzyme immunoassay (Thermo Scientific Multiskan FC, Thermo Fisher Scientific, USA), using IgA, IgM, IgE and IgG in serum, statistical processing was performed using Statistica 7.0.

RESULTS

In patients with CRS and IgE-mediated CRS, hyperactivation was revealed in both T- and B-cell immunity, manifested by an increase in the level of T-lymphocytes, NK-lymphocytes and B-lymphocytes. More pronounced disorders in the immune status are detected in patients with IgE — mediated CRS, there is a more pronounced activation of the T-cell immune link due to an increase in T-helper cells, T-killer/suppressor cells, an imbalance in their number is accompanied by a decrease in their ratio in the immunoregulatory index. Activation of the immune system in patients with CRS is also associated with an increase in the content of mature B-lymphocytes (CD19⁺), while only in patients with IgE-mediated CRS, hypergammaglobulinemia of classes A and M was detected.

CONCLUSION

Changes in the immune status indicate a violation of immune regulation, confirmed by the revealed correlations between the subpopulations of lymphocytes and immunoglobulins that implement the immune response in this condition. The greatest number of violations in the regulation is associated with mature T-lymphocytes in both CRS and IgE-mediated CRS, while only IgA fully retains its function.

Keywords:

chronic rhinusinusitis

cellular immunity

humoral immunity

Authors:

Smirnova O.V.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0003-3992-9207

Sinyakov A.A.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0002-4474-1893

Markina A.N.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0001-9539-4417

Parilova O.V.

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences

ORCID: 0000-0003-2948-9658

Received:

03.02.2023

Accepted:

24.04.2023

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References:

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