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Kostyushev D.S.

Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Zueva A.P.

Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia;
M.V. Lomonosov Moscow State University, Moscow, Russia

Brezgin S.A.

Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Lipatnikov A.D.

Central Research Institute of Epidemiology, Moscow, Russia;
D.I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

Simirskii V.N.

N.K. Koltzov Institute of Developmental Biology, Moscow, Russia

Glebe D.

Justus-Liebig University of Giessen, Institute of Medical Virology, Giessen, Germany

Volchkova E.V.

St. Petersburg State Pediatric Medical University of the Ministry of health of Russia, Saint-Petersburg, Russia

Shipulin G.A.

Federal State Budgetary Institution of Science Central Research Institute of Epidemiology, Russian Federal Consumer Rights Protection and Human Health Control Service, Moscow

Chulanov V.P.

Tsentral'nyĭ NII épidemiologii Rospotrebnadzora, Moskva

Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B

Authors:

Kostyushev D.S., Zueva A.P., Brezgin S.A., Lipatnikov A.D., Simirskii V.N., Glebe D., Volchkova E.V., Shipulin G.A., Chulanov V.P.

More about the authors

Journal: Therapeutic Archive. 2017;89(11): 21‑26

DOI: 10.17116/terarkh2017891121-26

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Table of contents

OVEREXPRESSION OF DNA-METHYLTRANSFERASES IN PERSISTENCY OF CCCDNA POOL IN CHRONIC HEPATITIS B
OVEREXPRESSION OF DNA-METHYLTRANSFERASES IN PERSISTENCY OF CCCDNA POOL IN CHRONIC HEPATITIS B

To cite this article:

Kostyushev DS, Zueva AP, Brezgin SA, et al. . Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B. Therapeutic Archive. 2017;89(11):21‑26. (In Russ.)
https://doi.org/10.17116/terarkh2017891121-26

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Abstract:

Aim. To define the role of DNA-methyltransferases of type 1 and type 3A in hepatitis B viral cycle. Materials and methods. Human hepatoma cells HepG2 with stable expression of 1.1-mer HBV genome were transfected with vectors encoding DNA-methyltransferase 1 (DNMT1), DNA-methyltransferase 3A (DNMT3A) or were co-transfected with these vectors. Total HBV DNA copy number, relative expression of pregenomic RNA (pgRNA), S-protein-encoding RNA (S-RNA) and cccDNA were analyzed by quantitative and semi-quantitative real-time PCR-analysis with TaqMan probes for assessment of DNMTs-mediated effects on HBV. Results. DNMT1 and DNMT3A suppress HBV transcription and replication, though to different magnitude. cccDNA pool is enlarged statistically significantly ≈2-fold (P<0.005) after transfection of DNMT3A, but is unaltered under DNMT1 treatment. Conclusion. DNMT3A regulates the size of cccDNA pool and is important for persistency of HBV infection.

Keywords:

hepatitis B virus (HBV)
chronic hepatitis B (CHB)
cccDNA
pgRNA
PCR
DNA-methyltransferases (DNMT)

Authors:

Kostyushev D.S.

Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Zueva A.P.

Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia;
M.V. Lomonosov Moscow State University, Moscow, Russia

Brezgin S.A.

Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

Lipatnikov A.D.

Central Research Institute of Epidemiology, Moscow, Russia;
D.I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

Simirskii V.N.

N.K. Koltzov Institute of Developmental Biology, Moscow, Russia

Glebe D.

Justus-Liebig University of Giessen, Institute of Medical Virology, Giessen, Germany

Volchkova E.V.

St. Petersburg State Pediatric Medical University of the Ministry of health of Russia, Saint-Petersburg, Russia

Shipulin G.A.

Federal State Budgetary Institution of Science Central Research Institute of Epidemiology, Russian Federal Consumer Rights Protection and Human Health Control Service, Moscow

Chulanov V.P.

Tsentral'nyĭ NII épidemiologii Rospotrebnadzora, Moskva

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