Determination of the organ and species of lung tissue microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay

Gusarov A.A.; Sidorov V.L.; Surikova N.E.; Yagmurov O.D.; Nagornov M.N.

doi:https://doi.org/10.17116/sudmed20266901147

Gusarov A.A.

Russian Center of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination;
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor

ORCID: 0000-0002-2214-895X

Surikova N.E.

Bureau of Forensic Medical Examination of the Department of Health of Moscow

ORCID: 0009-0005-0314-9569

Yagmurov O.D.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-0200-8474

Nagornov M.N.

Russian Center of Forensic Medical Expertise Ministry of Health of the Russian Federation

ORCID: 0000-0001-5077-2090

Determination of the organ and species of lung tissue microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay

Authors:

Gusarov A.A., Sidorov V.L., Surikova N.E., Yagmurov O.D., Nagornov M.N.

More about the authors

Journal: Forensic Medical Expertise. 2026;69(1): 47‑51

DOI: 10.17116/sudmed20266901147

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To cite this article:

Gusarov AA, Sidorov VL, Surikova NE, Yagmurov OD, Nagornov MN. Determination of the organ and species of lung tissue microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2026;69(1):47‑51. (In Russ.)
https://doi.org/10.17116/sudmed20266901147

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

OBJECTIVE

To determine the species belonging of lung tissue microparticles by total IgG using a quantitative enzyme-linked immunosorbent assay using the «IgG-total-ELISA-CHEMA» kit and to demonstrate the polymorphism of type I and II alveolocytes.

MATERIAL AND METHODS

Microparticles of lung tissue taken from corpses were studied. The study of these samples was carried out using the forensic cytological method and the method of quantitative enzyme immunoassay using the «IgG-general-ELISA-CHEMA» test kit.

RESULTS

The possibility of studying microparticles of lung tissue using ELISA has been established, optical density and total IgG concentrations have been determined, and optimal values for minimum and maximum calibration samples have been selected. Using the cytological method, the polymorphism of alveolocytes stained with fluorochromes and a neutral dye was demonstrated.

CONCLUSION

The data from the conducted high-tech research can be used to establish the species identity of microparticles of lung tissue found on instruments of injury.

Keywords:

microparticles of lung tissue

alveolocytes

species

quantitative enzyme-linked immunosorbent assay

Authors:

Gusarov A.A.

Russian Center of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination;
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor

ORCID: 0000-0002-2214-895X

Surikova N.E.

Bureau of Forensic Medical Examination of the Department of Health of Moscow

ORCID: 0009-0005-0314-9569

Yagmurov O.D.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-0200-8474

Nagornov M.N.

Russian Center of Forensic Medical Expertise Ministry of Health of the Russian Federation

ORCID: 0000-0001-5077-2090

Received:

20.02.2025

Accepted:

04.03.2025

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References:

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