Establishing the species of myocardial microparticles by human total IgG

Gusarov A.A.; Sidorov V.L.; Surikova N.E.; Yagmurov O.D.; Momynaliev K.T.

doi:https://doi.org/10.17116/sudmed20266903120

Gusarov A.A.

Russian Center of Forensic Medical Expertise, Moscow, Russia;
Sechenov First Moskow State Medical University, Moscow, Russia

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medicine, Saint Petersburg, Russia;
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor, Moscow, Russia

ORCID: 0000-0002-2214-895X

Surikova N.E.

Bureau of Forensic Medical Examination of the Moscow Health Department, Moscow, Russia

ORCID: 0009-0005-0314-9569

Yagmurov O.D.

Bureau of Forensic Medicine, Saint Petersburg, Russia

ORCID: 0000-0002-0200-8474

Momynaliev K.T.

All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor, Moscow, Russia

ORCID: 0000-0003-4656-1025

Establishing the species of myocardial microparticles by human total IgG

Authors:

Gusarov A.A., Sidorov V.L., Surikova N.E., Yagmurov O.D., Momynaliev K.T.

More about the authors

Journal: Forensic Medical Expertise. 2026;69(3): 20‑24

DOI: 10.17116/sudmed20266903120

Read: 335 times

Contact the author

Table of contents

To cite this article:

Gusarov AA, Sidorov VL, Surikova NE, Yagmurov OD, Momynaliev KT. Establishing the species of myocardial microparticles by human total IgG. Forensic Medical Expertise. 2026;69(3):20‑24. (In Russ.)
https://doi.org/10.17116/sudmed20266903120

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

OBJECTIVE

Establishing the species origin of microparticles of striated cardiac muscle tissue according to human total IgG by the method of quantitative solid-phase enzyme-linked immunosorbent assay using the IgG-total-ELISA-HEMA kit.

MATERIAL AND METHODS

Myocardial microparticles taken from the corpses of eight people. The samples were analyzed using the quantitative solid-phase ELISA method using the IgG-total-ELISA-HEMA test kit.

RESULTS

The optical density and concentration of total IgG in whole extracts were determined, and the optimal values of the minimum and maximum calibration samples were obtained: 0.082±0.015—2.581±0.017.

CONCLUSION

The obtained data can be used by experts of forensic biological units to establish the species affiliation of microparticles of striated cardiac muscle tissue found on trauma tools using the quantitative solid-phase ELISA method for human total IgG.

Keywords:

quantitative enzyme-linked immunosorbent assay

species

myocardial microparticles

optical density

calibration samples

Authors:

Gusarov A.A.

Russian Center of Forensic Medical Expertise, Moscow, Russia;
Sechenov First Moskow State Medical University, Moscow, Russia

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medicine, Saint Petersburg, Russia;
All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor, Moscow, Russia

ORCID: 0000-0002-2214-895X

Surikova N.E.

Bureau of Forensic Medical Examination of the Moscow Health Department, Moscow, Russia

ORCID: 0009-0005-0314-9569

Yagmurov O.D.

Bureau of Forensic Medicine, Saint Petersburg, Russia

ORCID: 0000-0002-0200-8474

Momynaliev K.T.

All-Russian Research and Testing Institute of Medical Equipment of Roszdravnadzor, Moscow, Russia

ORCID: 0000-0003-4656-1025

Received:

12.05.2025

Accepted:

22.05.2025

Close metadata

References:

Sidorov VL, Gusarov AA, Yagmurov OD. Modern expert algorithms for studying traces of blood, sperm and saliva on physical evidence. Bulletin of the Russian Society of specialists in medical and social expertise, rehabilitation and rehabilitation industry. 2017;4:70-81. (In Russ.).
Fetisov VA., Gusarov AA., Khabova ZS., Smirenin SA. Modern problems of injury research in publications of the journal “Forensic Medical Examination” (2000-2014). Forensic Medical Expertise. 2015; 58 (4): 56-62. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA. Methods of using quantitative enzyme immunoassay to establish the species affiliation of biological objects and the definitive detection of sperm in material evidence. Vestnik sudebnoy meditsiny. 2021;10(2):4-8. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA. Algoritm issledovaniya sledov krovi i vydelenij na veshchestvennyh dokazatel’stvah metodami kolichestvennogo immunofermentnogo analiza i kolorimetrii: metodicheskie rekomendacii. M.: RCFME; 2021. (In Russ.).
Gusarov AA, Sidorov VL, Yagmurov OD, Nagornov MN. Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2025;68(1):38-42. (In Russ.). https://doi.org/10.17116/sudmed20256801138
Gusarov AA. O neobhodimosti preobrazovaniya sistemy podgotovki ekspertnyh kadrov dlya sudebno-biologicheskih otdelenij GSEU. Aktual’nye voprosy sudebnoj mediciny i ekspertnoj praktiki na sovremennom etape: materialy Vserossijskoj nauchno-prakticheskoj konferencii s mezhdunarodnym uchastiem, posvyashchennoj 75-letiyu Rossijskogo centra sudebno-medicinskoj ekspertizy. Moskva. 17–20 oktyabrya 2006 goda. Pod redakciej V. A. Klevno. Moskva: RIO FGBU “Rossijskij centr sudebno-medicinskoj ekspertizy” Minzdrava Rossii, 2006; 73-74. (In Russ.).
Gusarov AA. Sposob vyyavleniya agglyutininov v usloviyah vliyaniya predmeta-nositelya. Sovershenstvovanie sudebno-medicinskoj ekspertizy v usloviyah reformirovaniya Vooruzhennyh Sil Rossijskoj Federacii, Moskva, 03 aprelya 2003 goda. Moskva: Burdenko, 2004; 199-200. (In Russ.).
Gusarov AA, Surikova NE. Metodika proizvodstva sudebno-citologicheskih issledovanij po delam o polovyh prestuplenijah. Uchebnoe posobie. Moskva; 2023. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA. Comparative characteristics of methods of studying the material evidence used for establishing the presence of blood and secretions in the russian federation and in foreign countries. Vestnik sudebnoy meditsiny. 2020;9(1):10-16. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Yagmurov OD. Ustanovlenie vidovoj prinadlezhnosti biologicheskih ob”ektov po IgG cheloveka s pomoshch’yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya medicinskaya tekhnologiya. M.: RCFME; 2011. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Cherepanova TV., Spiridonova OV., Yagmurov OD. Ustanovlenie nalichiya spermy na veshchestvennyh dokazatel’stvah po prostaticheskomu specificheskomu antigenu cheloveka s pomoshch’yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya medicinskaya tekhnologiya. M.: RCFME; 2011. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV. M. 2012. Ustanovlenie nalichiya spermy na veshchestvennyh dokazatel’stvah po kisloj fosfataze kolorimetricheskim metodom. Metodicheskie rekomendatsii. M.: RCFME;2012. (In Russ.).
Fokina EV, Gusarov AA. Forensic biological examination in road traffic accidents. Meditsinskaja ekspertiza i pravo. 2011;4:20-22. (In Russ.).
Gusarov AA, Fokina EV. A rare case of a car injury with a tree stump penetrating into the car interior. Meditsinskaja ekspertiza i pravo. 2011;3:52-53. (In Russ.).
Gusarov AA, Harlamov SG, Gurgenidze EV. Organizaciya otbora i issledovanie biologicheskogo materiala dlya ustanovleniya ego gruppovoj prinadlezhnosti pri massovom postuplenii neopoznannyh pogibshih. Istoriya, sovremennost’ i perspektivy sudebno-medicinskoj ekspertizy v Vooruzhyonnyh Silah Rossijskoj Federacii: Sbornik trudov Central’noj sudebno-medicinskoj laboratorii Ministerstva oborony Rossijskoj Federacii (CSML MO RF) k 100-letiyu M.I. Avdeeva, Moskva, 01 dekabrya 2001 goda. Ministerstvo oborony Rossijskoj Federacii, Central’naya sudebno-medicinskaya laboratoriya. Moskva: Nauchno-metodicheskij centr Glavnogo voennogo klinicheskogo gospitalya im. N.N. Burdenko, 2001; 51-53 (In Russ.).
Gusarov AA, Sidorov VL, Yagmurov OD, Surikova NE. Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2024;67(6):21-24. (In Russ.). https://doi.org/10.17116/sudmed20246706121
Gusarov AA, Sidorov VL, Surikova NE, Yagmurov OD. Determination of tissular and species membership of hyaline cartilage microparticles by forensic cytology and enzyme-linked immunosorbent assay methods. Forensic Medical Expertise. 2025;68(2):21-24. (In Russ.). https://doi.org/10.17116/sudmed20256802121
Fedorovcev AL, Revnitskaja LA, Koroleva EI, Jedelev NS. Sudebno-meditsinskie citologicheskie isledovanija sledov na veshhestvennyh dokazatel’stvah. Monografija. Nizhnij Novgorod; 2009. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Application of quantitative methods for studying traces of blood and secretions on material evidence during forensic biological examinations. Vestnik sudebnoy meditsiny. 2020. 9 (2): 28-34. (In Russ.).