Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay

Gusarov A.A.; Sidorov V.L.; Yagmurov O.D.; Surikova N.E.

doi:https://doi.org/10.17116/sudmed20246706121

Gusarov A.A.

Russian Center of Forensic Medical Expertise;
th Main State Center for Forensic and Criminalistic Examinations

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-0200-8474

Surikova N.E.

Bureau of Forensic Medical Examination of the Moscow City Health Department

ORCID: 0009-0005-0314-9569

Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay

Authors:

Gusarov A.A., Sidorov V.L., Yagmurov O.D., Surikova N.E.

More about the authors

Journal: Forensic Medical Expertise. 2024;67(6): 21‑24

DOI: 10.17116/sudmed20246706121

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To cite this article:

Gusarov AA, Sidorov VL, Yagmurov OD, Surikova NE. Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2024;67(6):21‑24. (In Russ.)
https://doi.org/10.17116/sudmed20246706121

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

OBJECTIVE

Improving the way to establish the species belonging of muscle tissue fragments and particles using the quantitative solid-phase enzyme-linked immunosorbent assay method.

MATERIAL AND METHODS

We studied human muscle tissue fragments and particles (sampled 2—4 weeks before) and also dried extracts from the blood of certain animals, such as dogs, birds, pigs, and cats. The samples were studied using quantitative enzyme immunoassay with the domestic kit «IgG-total-ELISA-HEMA».

RESULTS

The study confirmed the method’s sensitivity and specificity. We determined the optical density and concentration total IgG indicative values and selected the optimal values of the minimum and maximum calibration samples: 0.076±0.014—2.623±0.351.

CONCLUSION

Experts from forensic biological units can use the obtained data when establishing the species belonging of muscle tissue particles using a quantitative solid-phase enzyme-linked immunosorbent assay method.

Keywords:

muscle tissue particles

quantitative solid-phase enzyme-linked immunosorbent assay method

species belonging

Authors:

Gusarov A.A.

Russian Center of Forensic Medical Expertise;
th Main State Center for Forensic and Criminalistic Examinations

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-0200-8474

Surikova N.E.

Bureau of Forensic Medical Examination of the Moscow City Health Department

ORCID: 0009-0005-0314-9569

Received:

29.01.2024

Accepted:

13.03.2024

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