Currently, there is a constant expansion of the range of narcotic substances that differ from each other by a small structural fragment. The option of determining narcotic substances using test panels is widespread due to its rapidity and high specificity through the use of immunochemical reactions. The purpose of the study was to optimize the determination of morphine and amphetamine in bioobjects (synthetic and natural urine) using immunochromatographic analysis with new test panels and to select the optimal conditions for mass analysis. Test panels were used to detect the presence of amphetamine and morphine. For color recording of the results of analysis, colloidal gold nanoparticles were used. The principle of operation of these test panels is described. The sensitivity of the test panels is such that it is possible to avoid false-positive results. It was found that with the help of test panels it is possible to determine narcotic substances in a concentration lower than stated in the instructions (300 ng/ml). The actual detection limit for amphetamine was 75 ng/ml and morphine 100 ng/ml. The analytical characteristics of the developed metho-dology were determined: detection limit, precision, truth and specificity. The specificity was proved by conducting ICA to detect the presence of cross-reactions of test systems to amphetamine and morphine using structural analogues — adrenaline 1000 and codeine 300, respectively. The study did not receive false positive results for these molecules using the proposed test panels. Immunochromatographic test systems are optimal for drug detection, especially when conducting mass studies.