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Seredin P.V.

Voronezh State University

Ippolitov Yu.A.

Voronezh State Medical University named after N.N. Burdenko

Peshkov Y.A.

Voronezh State University

Goloshchapov D.L.

Voronezh State University

Ippolitov I.Yu.

Voronezh State Medical University named after N.N. Burdenko

Avraamova O.G.

Central Research Institute of Dentistry and Maxillofacial Surgery

Distinctions in molecular composition of the dental biofilm in a dependence of method of exo/endogeneous caries prevention and cariogenic condition of a patient

Authors:

Seredin P.V., Ippolitov Yu.A., Peshkov Y.A., Goloshchapov D.L., Ippolitov I.Yu., Avraamova O.G.

More about the authors

Journal: Stomatology. 2024;103(5): 5‑12

DOI: 10.17116/stomat20241030515

Views: 468

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Table of contents

DISTINCTIONS IN MOLECULAR COMPOSITION OF THE DENTAL BIOFILM IN A DEPENDENCE OF METHOD OF EXO/ENDOGENEOUS CARIES PREVENTION AND CARIOGENIC CONDITION OF A PATIENT
DISTINCTIONS IN MOLECULAR COMPOSITION OF THE DENTAL BIOFILM IN A DEPENDENCE OF METHOD OF EXO/ENDOGENEOUS CARIES PREVENTION AND CARIOGENIC CONDITION OF A PATIENT

To cite this article:

Seredin PV, Ippolitov YuA, Peshkov YA, Goloshchapov DL, Ippolitov IYu, Avraamova OG. Distinctions in molecular composition of the dental biofilm in a dependence of method of exo/endogeneous caries prevention and cariogenic condition of a patient. Stomatology. 2024;103(5):5‑12. (In Russ.)
https://doi.org/10.17116/stomat20241030515

Подписка 2025-2 (Stomatology)
 
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OBJECTIVE

Comparative study of changes in the secondary structure of dental biofilm proteins in people with cariesogenic and cariesoprotective status using synchrotron IR microspectroscopy.

MATERIALS AND METHODS

Dental biofilm samples from 50 patients without caries (group 1) and with carious lesions of tooth enamel (group 2) were studied. The molecular composition of biofilms was studied using IR microspectroscopy. Changes in the secondary structure of dental biofilm proteins were evaluated based on deconvolution of Amide I or Amide II amide bands. using the analysis of variance. Patients of both groups took tablets containing a mineral complex with calcium glycerophosphate

RESULTS

Based on the deconvolution of the IR spectral profile of the Amide I and Amide II bands, the changes occurring in the secondary structure of dental biofilm proteins have been studied. Using a large set of spectra, it was shown that the prediction of the secondary structure of the biofilm protein network is influenced by both the cariesogenic situation in the oral cavity (with initial carious lesions of the enamel) and the fact that patients use a modulator — calcium glycerophosphate (a tableted mineral complex with calcium glycerophosphate). Significant intra-group and intergroup differences in the secondary structure of dental biofilm proteins were established for patients in normal and carisogenic situations in the mouth, including after the use of a tableted mineral complex with calcium glycerophosphate. This allowed us to give a mathematical assessment of shifts in the secondary structure of proteins depending on the activity of caries and external modulation.

CONCLUSION

The results obtained can form the basis of a technique for spectroscopic diagnosis of changes (shifts) in the oral microbiome leading to the development of the carious process, as well as become the basis for choosing the optimal therapeutic ways to treat enamel caries, including through preventive measures aimed at restoring the microflora of the patient’s oral cavity.

Keywords:

dental biodilm
caries
exo- and endogeneous prevention
IR-microspectroscopy

Authors:

Seredin P.V.

Voronezh State University

Ippolitov Yu.A.

Voronezh State Medical University named after N.N. Burdenko

Peshkov Y.A.

Voronezh State University

Goloshchapov D.L.

Voronezh State University

Ippolitov I.Yu.

Voronezh State Medical University named after N.N. Burdenko

Avraamova O.G.

Central Research Institute of Dentistry and Maxillofacial Surgery

Received:

17.11.2023

Accepted:

01.01.2024

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