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Vasilyev A.V

Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia;

Laboratory of Stem Cell Genetics of the Medical Genetics Research Center, Moscow, Russia;

Peoples' Friendship University of Russia, Moscow, Russia

Kuznetsova V.S.

Research Centre for Medical Genetics;
Central Research Institute of Dentistry and Maxillofacial Surgery

Galitsyna E.V.

Research Centre for Medical Genetics, Moscow, Russia

Bukharova T.B.

Research Centre for Medical Genetics

Osidak E.O.

Imtek Ltd., Moscow, Russia

Fatkhudinova N.L.

Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia

Leonov G.E.

Research Centre for Medical Genetics, Moscow, Russia

Babichenko I.I.

Peoples’ Friendship University of Russia;
Central Research Institute of Dentistry and Maxillofacial Surgery

Domogatsky S.P.

Institute of Experimental Cardiology of the National Medical Research Center of Cardiology, Moscow, Russia

Goldshtein D.V.

Laboratory of Stem Cell Genetics of the Medical Genetics Research Center, Moscow, Russia

Kulakov A.A.

Central Research Institute of Dentistry and Maxillofacial Surgery

Biocompatibility and osteoinductive properties of collagen and fibronectin hydrogel impregnated with rhBMP-2

Authors:

Vasilyev A.V, Kuznetsova V.S., Galitsyna E.V., Bukharova T.B., Osidak E.O., Fatkhudinova N.L., Leonov G.E., Babichenko I.I., Domogatsky S.P., Goldshtein D.V., Kulakov A.A.

More about the authors

Journal: Stomatology. 2019;98(6‑2): 5‑11

DOI: 10.17116/stomat2019980625

Views: 2585

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Table of contents

BIOCOMPATIBILITY AND OSTEOINDUCTIVE PROPERTIES OF COLLAGEN AND FIBRONECTIN HYDROGEL IMPREGNATED WITH RHBMP-2
BIOCOMPATIBILITY AND OSTEOINDUCTIVE PROPERTIES OF COLLAGEN AND FIBRONECTIN HYDROGEL IMPREGNATED WITH RHBMP-2

To cite this article:

Vasilyev AV, Kuznetsova VS, Galitsyna EV, et al. . Biocompatibility and osteoinductive properties of collagen and fibronectin hydrogel impregnated with rhBMP-2. Stomatology. 2019;98(6‑2):5‑11. (In Russ.)
https://doi.org/10.17116/stomat2019980625

Подписка 2025-2 (Stomatology)
 
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The study aimed to demonstrate the biocompatibility and osteoinductive properties of a hydrogel based on highly purified collagen and fibronectin impregnated with rhBMP-2. In vitro and in vivo experiments have shown that the minimum effective dosage of rhBMP-2 is 10 μg/ml. The cytocompatibility of the collagen-fibronectin gel was determined using MTT test and staining with PKH-26. There was no inflammation reaction when the material was subcutaneously implanted in rats (n=30) in vivo. The collagen-fibronectin hydrogel containing 10 μg/ml rhBMP-2 showed high osteogenic properties. By the end of 28 days 8±4% of its volume was replaced by newly formed bone tissue in case of subcutaneous implantation, 17±10% in intramuscular implantation and 26±11% in intraosseous implantation in the calvarial critical-size. The optimal combination of biocompatible and osteogenic properties of collagen-fibronectin hydrogel impregnated with BMP-2 allows us to consider it as a promising basis for creating the new generation of osteoplastic materials for dentistry.

Keywords:

osteoplastic material
BMP-2
collagen-fibronectin hydrogel
osteoinduction

Authors:

Vasilyev A.V

Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia;

Laboratory of Stem Cell Genetics of the Medical Genetics Research Center, Moscow, Russia;

Peoples' Friendship University of Russia, Moscow, Russia

Kuznetsova V.S.

Research Centre for Medical Genetics;
Central Research Institute of Dentistry and Maxillofacial Surgery

Galitsyna E.V.

Research Centre for Medical Genetics, Moscow, Russia

Bukharova T.B.

Research Centre for Medical Genetics

Osidak E.O.

Imtek Ltd., Moscow, Russia

Fatkhudinova N.L.

Central Research Institute of Dentistry and Maxillofacial Surgery, Moscow, Russia

Leonov G.E.

Research Centre for Medical Genetics, Moscow, Russia

Babichenko I.I.

Peoples’ Friendship University of Russia;
Central Research Institute of Dentistry and Maxillofacial Surgery

Domogatsky S.P.

Institute of Experimental Cardiology of the National Medical Research Center of Cardiology, Moscow, Russia

Goldshtein D.V.

Laboratory of Stem Cell Genetics of the Medical Genetics Research Center, Moscow, Russia

Kulakov A.A.

Central Research Institute of Dentistry and Maxillofacial Surgery

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