Objective — to create a novel cell engineering construction to improve implantation materials used to restore fascial defects of the pelvic floor in patients with different types of genital prolapse. Material and methods. 2×3×1-mm skin samples were taken from a healthy volunteer to have dermal fibroblasts cultured in special media. Mesh implants for genital organ prolapse surgery, the samples of which third-passage human dermal fibroblasts were applied to, were used as a matrix. A set of meshes with cells were incubated under humid conditions at 37 ºC and microscopically examined. Results. Closure of wells with cells started on days 10—14 after their inoculation on the mesh. Closure of the bulk of the wells with a multilayer cellular structure was observed at 30 days of cultivation and that of all mesh wells was on 60 days. Conclusion. The proposed cell engineering construction based on collagen synthesis and fibrous tissue intergrowth on the mesh implants may lead to the stronger connection of the mesh with the adjacent tissues than synthetic prostheses and may improve the results of surgical treatment. The authors declare no conflicts of interest.