Study of the impact of smoking as a risk factor for atherosclerosis on the level of DNA methylation

Kiseleva A.V.

European Medical Centre. Moscow, Russia

Khlebus E.Yu.

National Research Center for Preventive Medicine, Ministry of Health of Russia ,Moscow, Russia, 101990

Ershova A.I.

Department of Hospital Surgery;
Institute of Continuous Media Mechanics, Ural Branch of RAS, Perm, Russia

Shakhnovich P.G.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Tkachenko K.N.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Makiev R.G.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Efimov S.V.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Cherkashin D.V.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Meshkov A.N.

The national medical research center of preventive, Petroverigsky lane 10 page 3, Moscow, 101990

Boytsov S.A.

National Research Centre for Preventive Medicine, Moscow, Russia

Study of the impact of smoking as a risk factor for atherosclerosis on the level of DNA methylation

Authors:

Kiseleva A.V., Khlebus E.Yu., Ershova A.I., Shakhnovich P.G., Tkachenko K.N., Makiev R.G., Efimov S.V., Cherkashin D.V., Meshkov A.N., Boytsov S.A.

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Journal: Russian Journal of Preventive Medicine. 2015;18(6): 66‑70

DOI: 10.17116/profmed201518666-70

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To cite this article:

Kiseleva AV, Khlebus EYu, Ershova AI, et al. . Study of the impact of smoking as a risk factor for atherosclerosis on the level of DNA methylation. Russian Journal of Preventive Medicine. 2015;18(6):66‑70. (In Russ.)
https://doi.org/10.17116/profmed201518666-70

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Objective - to search for associations between smoking and genomic methylation level. Patients and methods. The investigation enrolled 162 patients (60 smokers 102 nonsmokers). The genomic methylation levels in the patients were investigated using Agilent human methylation microarrays; there were 235,336 examined CpG sites after removing X and Y chromosome sites. Fifteen patients were excluded from the analysis after using the main component method. Both a whole-genome methylation analysis and an additional subanalysis of 2038 CpG sites in 119 genes previously associated with smoking were performed. Results. Comparison of whole-genome methylation levels showed that the groups significantly differed in one CpG site in the SIX1 gene (p=9.39E-08, padj=0.0221). That of methylation levels in the smoking-related genes indicated that significant differences were found for two CpS sites in the ABTB1 (p=3.23E-05, padj=0.0458) and NAV2 (p=4.50E-05, padj=0.0458) genes. Conclusion. This study has demonstrated for the first time that there is a significant association of methylation of a CpG site in the SIX1 gene with smoking. In addition, there is evidence that smoking correlates with methylation of the CpG sites in the ABTB1 and NAV2 genes.

Keywords:

methylation

smoking

DNA microarray

EWAS

Authors:

Kiseleva A.V.

European Medical Centre. Moscow, Russia

Khlebus E.Yu.

National Research Center for Preventive Medicine, Ministry of Health of Russia ,Moscow, Russia, 101990

Ershova A.I.

Department of Hospital Surgery;
Institute of Continuous Media Mechanics, Ural Branch of RAS, Perm, Russia

Shakhnovich P.G.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Tkachenko K.N.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Makiev R.G.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Efimov S.V.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Cherkashin D.V.

S.M. Kirov Military Medical Academy, Ministry of Defense of the Russian Federation, Saint Petersburg, Russia, 194044

Meshkov A.N.

The national medical research center of preventive, Petroverigsky lane 10 page 3, Moscow, 101990

Boytsov S.A.

National Research Centre for Preventive Medicine, Moscow, Russia

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