The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Vaĭner A.S.

Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny Sibirskogo otdeleniia RAN, Novosibirsk;
Novosibirskiĭ gosudarstvennyĭ universitet

Boiarskikh U.A.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Voronina E.N.

Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny SO RAN, Novosibirsk

Shirshova A.N.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Mishukova O.V.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Osadchuk L.V.

FGBU "Institut tsitologii i genetiki" SO RAN, Novosibirsk

Filipenko M.L.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia;
Novosibirsk State University, Novosibirsk, Russia

The diagnostic system development for the detection of azf-microdeletions with multiplex real-time pcr

Authors:

Vaĭner A.S., Boiarskikh U.A., Voronina E.N., Shirshova A.N., Mishukova O.V., Osadchuk L.V., Filipenko M.L.

More about the authors

Read: 896 times


To cite this article:

Vaĭner AS, Boiarskikh UA, Voronina EN, Shirshova AN, Mishukova OV, Osadchuk LV, Filipenko ML. The diagnostic system development for the detection of azf-microdeletions with multiplex real-time pcr. Russian Journal of Human Reproduction. 2013;(2):74‑77. (In Russ.)

Recommended articles:
M. geni­talium-infection. Correlation between pathogen’s DNA concentration and clinical picture of disease. Russian Journal of Clinical Dermatology and Vene­reology. 2025;(4):446-452

References:

  1. Ali S., Hasnain S.E. Genomics of the human Y-chromosome. 1. Association with male infertility. Gene 2003; 321: 25-37.
  2. Simoni M., Bakker E., Eurlings M.C., Matthijs G., Moro E., Müller C.R., Vogt P.H. Laboratory guidelines for molecular diagnosis of Y-chromosomal microdeletions. Intern J Androl 1999; 22: 5: 292-299.
  3. Tiepolo L., Zuffardi O. Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long-arm. Human Gen 1976; 34: 2: 119-124.
  4. Vogt P.H., Edelmann A., Kirsch S., Henegariu O., Hirschmann P., Kiesewetter F., Köhn F.M., Schill W.B., Farah S., Ramos C., Hartmann M., Hartschuh W., Meschede D., Behre H.M., Castel A., Nieschlag E., Weidner W., Gröne H.J., Jung A., Engel W., Haidl G. Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11. Human Mol Genet 1996; 5: 7: 933-943.
  5. Ferlin A., Moro E., Rossi A., Dallapiccola B., Foresta C. The human Y chromosome's azoospermia factor b (AZFb) region: sequence, structure, and deletion analysis in infertile men. J Med Genet 2003; 40: 1: 18-24.
  6. Holland P.M., Abramson R.D., Watson R., Gelfand D.H. Detection of specific polymerase chain reaction product by utilizing the 5'-3' exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings. Nat Acad Sci (USA) 1991; 88: 16: 7276-7280.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.