The diagnostic system development for the detection of azf-microdeletions with multiplex real-time pcr

Vaĭner A.S.

Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny Sibirskogo otdeleniia RAN, Novosibirsk;
Novosibirskiĭ gosudarstvennyĭ universitet

Boiarskikh U.A.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Voronina E.N.

Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny SO RAN, Novosibirsk

Shirshova A.N.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Mishukova O.V.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Osadchuk L.V.

FGBU "Institut tsitologii i genetiki" SO RAN, Novosibirsk

Filipenko M.L.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia;
Novosibirsk State University, Novosibirsk, Russia

The diagnostic system development for the detection of azf-microdeletions with multiplex real-time pcr

Authors:

Vaĭner A.S., Boiarskikh U.A., Voronina E.N., Shirshova A.N., Mishukova O.V., Osadchuk L.V., Filipenko M.L.

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Journal: Russian Journal of Human Reproduction. 2013;(2): 74‑77

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To cite this article:

Vaĭner AS, Boiarskikh UA, Voronina EN, Shirshova AN, Mishukova OV, Osadchuk LV, Filipenko ML. The diagnostic system development for the detection of azf-microdeletions with multiplex real-time pcr. Russian Journal of Human Reproduction. 2013;(2):74‑77. (In Russ.)

Подписка 2025-2 (Russian Journal of Human Reproduction)

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Microdeletions of AZFa, AZFb and AZFc regions on the long arm of the Y-chromosome cause azoo- and oligozoospermia. We developed the diagnostic system for detection of these microdeletions with the help of two tetraplex real-time polymerase chain reactions.

Keywords:

male infertility

AZF region

PCR

TaqMan

diagnostic system

Authors:

Vaĭner A.S.

Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny Sibirskogo otdeleniia RAN, Novosibirsk;
Novosibirskiĭ gosudarstvennyĭ universitet

Boiarskikh U.A.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Voronina E.N.

Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny SO RAN, Novosibirsk

Shirshova A.N.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Mishukova O.V.

FGBUN "Institut khimicheskoĭ biologii i fundamental'noĭ meditsiny" SO RAN

Osadchuk L.V.

FGBU "Institut tsitologii i genetiki" SO RAN, Novosibirsk

Filipenko M.L.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia;
Novosibirsk State University, Novosibirsk, Russia

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