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Pyankov S.A.

State Scientific Center of Virology and Biotechnology “Vector”

Shulgina I.S.

State Scientific Center of Virology and Biotechnology “Vector”

Rybel A.V.

State Scientific Center of Virology and Biotechnology “Vector”

Maksyutov A.Z.

State Scientific Center of Virology and Biotechnology “Vector”

Tyurin V.Yu.

State Scientific Center of Virology and Biotechnology “Vector”

Drachkova I.A.

State Scientific Center of Virology and Biotechnology “Vector”

Tregubchak T.V.

State Scientific Center of Virology and Biotechnology “Vector”

Bauer T.V.

State Scientific Center of Virology and Biotechnology “Vector”

Ovchinnikova A.S.

State Scientific Center of Virology and Biotechnology “Vector”

Odnoshevskiy D.A.

State Scientific Center of Virology and Biotechnology “Vector”

Kabanov A.S.

State Scientific Center of Virology and Biotechnology “Vector”

Bodnev S.A.

State Scientific Center of Virology and Biotechnology “Vector”

Pyankov O.V.

State Scientific Center of Virology and Biotechnology “Vector”

Agafonov A.P.

State Scientific Center of Virology and Biotechnology “Vector”

Application of A29L protein specific monoclonal antibodies a-A29L_MPoxV for monkeypox diagnosis

Authors:

Pyankov S.A., Shulgina I.S., Rybel A.V., Maksyutov A.Z., Tyurin V.Yu., Drachkova I.A., Tregubchak T.V., Bauer T.V., Ovchinnikova A.S., Odnoshevskiy D.A., Kabanov A.S., Bodnev S.A., Pyankov O.V., Agafonov A.P.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2023;41(4): 31‑37

DOI: 10.17116/molgen20234104131

Views: 704

Downloaded: 49

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Table of contents

To cite this article:

Pyankov SA, Shulgina IS, Rybel AV, et al. . Application of A29L protein specific monoclonal antibodies a-A29L_MPoxV for monkeypox diagnosis. Molecular Genetics, Microbiology and Virology. 2023;41(4):31‑37. (In Russ.)
https://doi.org/10.17116/molgen20234104131

Подписка 2025 (Molecular Genetics, Microbiology and Virology)
 
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INTRODUCTION

The spread of the disease caused by monkeypox virus (MPox) since 2022 has shown the urgency of developing countermeasures. The development of modern methods of clinical laboratory diagnostics of MPox contributes to this. Enzyme-linked immunosorbent assay (ELISA) is an accessible and sensitive platform for developing diagnostic tools. Detection of MPox antigens using ELISA kits based on monoclonal antibodies (MAbs) is promising due to quick time of analysis and minimal requirements for sample preparation.

MATHERIAL AND METHODS

We have developed and deposited two strains of Escherichia coli that produce recombinant proteins. Mice were immunized the AgPOX protein contains unique antigenic sequences of MPox. The Trx+A29 protein for selecting MAb producers includes the original amino acid sequence A29L. The absence of antibody crossover to Trx protein and native preparations of variola virus and vaccinia virus tested by ELISA. As a result of hybridization of splenocytes from immunized mice, MAb producers were obtained.

RESULTS

15 MAb-producing hybridomas were selected based on ELISA results with three specific MPox antigens and three non-specific ones. Three hybridomas were selected for deposit according to the productivity criteria. The possibility of detecting by its MAbs of the native MPox antigen at various concentrations was tested and method sensitivity was determined. The MAbs a-A29L_MPoxV of three hybridomas detected the native antigen MPox at a concentration of 102 PFU/ml. It is likely that the method is even more sensitive when selecting analysis conditions.

CONCLUSION

Based on labeled MAbs a-A29L_MPoxV, it is possible to develop a sensitive and specific indirect two-step ELISA kit for immunodiagnostics of MPox.

Keywords:

monkeypox virus
enzyme-linked immunosorbent assay
antigen
strain
hybridoma
monoclonal antibody

Authors:

Pyankov S.A.

State Scientific Center of Virology and Biotechnology “Vector”

Shulgina I.S.

State Scientific Center of Virology and Biotechnology “Vector”

Rybel A.V.

State Scientific Center of Virology and Biotechnology “Vector”

Maksyutov A.Z.

State Scientific Center of Virology and Biotechnology “Vector”

Tyurin V.Yu.

State Scientific Center of Virology and Biotechnology “Vector”

Drachkova I.A.

State Scientific Center of Virology and Biotechnology “Vector”

Tregubchak T.V.

State Scientific Center of Virology and Biotechnology “Vector”

Bauer T.V.

State Scientific Center of Virology and Biotechnology “Vector”

Ovchinnikova A.S.

State Scientific Center of Virology and Biotechnology “Vector”

Odnoshevskiy D.A.

State Scientific Center of Virology and Biotechnology “Vector”

Kabanov A.S.

State Scientific Center of Virology and Biotechnology “Vector”

Bodnev S.A.

State Scientific Center of Virology and Biotechnology “Vector”

Pyankov O.V.

State Scientific Center of Virology and Biotechnology “Vector”

Agafonov A.P.

State Scientific Center of Virology and Biotechnology “Vector”

Received:

28.09.2023

Accepted:

13.10.2023

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