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Khokhlova O.E.

State Research Center for Applied Microbiology & Biotechnology;
Pushchino State Institute of Natural Science

Vladimirov I.V.

Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Kozlov R.S.

Smolensk State Medical University

Lazareva I.V.

Children’s Research and Clinical Center for Infectious Diseases

Edelstein M.V.

Smolensk state medical university

Larionova I.A.

Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Molodtsova A.V.

Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Avdeeva V.A.

State Research Center for Applied Microbiology & Biotechnology

Fursova N.K.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Sidorenko S.V.

Far Eastern State Medical University

Molecular-genetic mechanisms of resistance to antibiotic of the pathogens in patients with thermal burns and infection

Authors:

Khokhlova O.E., Vladimirov I.V., Kozlov R.S., Lazareva I.V., Edelstein M.V., Larionova I.A., Molodtsova A.V., Avdeeva V.A., Fursova N.K., Sidorenko S.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(4): 22‑29

DOI: 10.17116/molgen20224004122

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Table of contents

MOLECULAR-GENETIC MECHANISMS OF RESISTANCE TO ANTIBIOTIC OF THE PATHOGENS IN PATIENTS WITH THERMAL BURNS AND INFECTION
MOLECULAR-GENETIC MECHANISMS OF RESISTANCE TO ANTIBIOTIC OF THE PATHOGENS IN PATIENTS WITH THERMAL BURNS AND INFECTION

To cite this article:

Khokhlova OE, Vladimirov IV, Kozlov RS, et al. . Molecular-genetic mechanisms of resistance to antibiotic of the pathogens in patients with thermal burns and infection. Molecular Genetics, Microbiology and Virology. 2022;40(4):22‑29. (In Russ.)
https://doi.org/10.17116/molgen20224004122

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Infectious complications caused by antibiotic-resistant strains in burn injury patients account for 30—75% of all deaths. For optimal clinical control important to know the susceptibility profile, the mechanisms of antimicrobial resistance and the predominant genetic lineage.

THE AIMED

At studying the mechanisms of antibiotic resistance of the main causative agents of pyoinflammatory complications in patients with burns.

MATERIALS AND METHODS

In 2011—2017 were examined 90 patients with deep burns who were treated at the Regional Clinical Hospital. Materials investigated by bacteriological method, MALDI-TOF. Antibiotic sensitivity was studied by methods: disk-diffusion; PCR; E-test; serial dilutions in Muller-Hinton broth. Genotyping and determination of resistance mechanisms: PCR, sequencing.

RESULTS

The prevalence of a mixed flora of multi- and extremely resistant pathogens, representatives of the ESKAPE group, has been established. 66.7% of P. aeruginosa strains (n=9) had blaVIM, 22.2% of strains — blaCTX-M. 50% of A. baumannii strains (n=8) — blaOXA-24-like, 25% — blaOXA-23-like, 100% — blaOXA-51-like, ompA, adeR. 50% of K. pneumonia strains (n=2) had blaTEM and blaCTX-M, 100% — blaSHV, ompK36. MRSA PVL- isolates (n=10), belong to the ST239;spat037;SCCmecIIIA;tst variant, had the aacA-aphD, aadD resistance genes; ermA; tetM; cat (plasmid 2.5 kb); mutations were revealed in GyrA-Ser84Leu and GrlA-Ser80Phe, as well as in rpoB-His481Asn, Ile527Met.

CONCLUSION

The distribution of P. aeruginosa, A. baumannii and K. pneumoniae strains with carbapenemases and other mechanisms among burns patients was presented. MRSA strains are due to the presence of mutations in chromosomal genes and had resistance genes.

Keywords:

burn
MRSA
P.aeruginosa
A.baumannii
K.pneumoniae
antibiotic resistance
molecular genetic mechanisms

Authors:

Khokhlova O.E.

State Research Center for Applied Microbiology & Biotechnology;
Pushchino State Institute of Natural Science

Vladimirov I.V.

Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Kozlov R.S.

Smolensk State Medical University

Lazareva I.V.

Children’s Research and Clinical Center for Infectious Diseases

Edelstein M.V.

Smolensk state medical university

Larionova I.A.

Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Molodtsova A.V.

Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

Avdeeva V.A.

State Research Center for Applied Microbiology & Biotechnology

Fursova N.K.

State Research Center for Applied Microbiology and Biotechnology Rospotrebnadzor

Sidorenko S.V.

Far Eastern State Medical University

Received:

14.10.2021

Accepted:

30.04.2022

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