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Yanovich O.O.

The Republican Research and Practical Center for Epidemiology and Microbiology

Titov L.P.

The Republican Research and Practical Center for Epidemiology and Microbiology

Doroshko M.V.

Medical center «Nordin»

Genetic diversity and functional status of oipA GENE Helicobacter pylori, its association with island of pathogenicity genes in patients with gastroduodenal diseases in the Republic of Belarus

Authors:

Yanovich O.O., Titov L.P., Doroshko M.V.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(4): 9‑13

DOI: 10.17116/molgen2022400419

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To cite this article:

Yanovich OO, Titov LP, Doroshko MV. Genetic diversity and functional status of oipA GENE Helicobacter pylori, its association with island of pathogenicity genes in patients with gastroduodenal diseases in the Republic of Belarus. Molecular Genetics, Microbiology and Virology. 2022;40(4):9‑13. (In Russ.)
https://doi.org/10.17116/molgen2022400419

 
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AIM

The aim of this study was to investigate the variability and functional status of the oipA gene H. pylori by using a sequencing method, to evaluate the association of oipA functionality with cagPAI genes and clinical outcomes.

MATERIALS AND METHODS

The object of the study was 84 samples of H. pylori DNA isolated from antrum biopsies of the gastric mucosa from patients with gastroduodenal diseases. The presence of cagPAI genes were determined by PCR. oipA gene status was determined using sequencing reactions.

RESULTS

Our results revealed that 79.8% of H. pylori samples have the oipA gene status «on», and the remaining 20.2% of the samples had a non-functional gene status. The oipA gene «on» status was found to be statistically associated with an increased risk of developing duodenal ulcer compared with superficial gastritis (p<0.003, OR=14.5). However, no significant association was observed between the functional oipA status and atrophy gastritis (p>0.05). The study showed a high frequency of detection of pathogenicity island genes among H. pylori DNA samples with the oipA gene status «on». There is a significant correlation between the oipA «on» gene and the presence of all studied cagPAI genes; the highest correlation was found for the cagT and cagM genes.

CONCLUSION

The link between functional oipA and other virulence factors encoded by the pathogenicity island cagPAI is to be important in the pathogenesis of H. pylori and needs further studies.

Keywords:

Helicobacter pylori
oipA
cag PAI
gastric disorders

Authors:

Yanovich O.O.

The Republican Research and Practical Center for Epidemiology and Microbiology

Titov L.P.

The Republican Research and Practical Center for Epidemiology and Microbiology

Doroshko M.V.

Medical center «Nordin»

Received:

12.07.2021

Accepted:

08.02.2022

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