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Kubanov A.A.

State Research Center of Dermatovenerology and Cosmetology of the Ministry of Healthcare of the Russian Federation

Solomka V.S.

State Research Center of Dermatovenerology and Cosmetology

Shpilevaya M.V.

State Research Center of Dermatovenerology and Cosmetology

Verbenko D.A.

State Research Center of Dermatovenerology and Cosmetology

Deryabin D.G.

State Research Center of Dermatovenerology and Cosmetology

Kandinov I.D.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Dementieva E.I.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Gryadunov D.A.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Shaskolskiy B.L.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Diversity of ng-mast and mlst sequence types in Russian clinical isolates of Neisseria gonorrhoeae carrying the «mosaic» allele of the penA gene

Authors:

Kubanov A.A., Solomka V.S., Shpilevaya M.V., Verbenko D.A., Deryabin D.G., Kandinov I.D., Dementieva E.I., Gryadunov D.A., Shaskolskiy B.L.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(4): 14‑21

DOI: 10.17116/molgen20224004114

Read: 1308 times

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Table of contents

DIVERSITY OF NG-MAST AND MLST SEQUENCE TYPES IN RUSSIAN CLINICAL ISOLATES OF NEISSERIA GONORRHOEAE CARRYING THE «MOSAIC» ALLELE OF THE PENA GENE
DIVERSITY OF NG-MAST AND MLST SEQUENCE TYPES IN RUSSIAN CLINICAL ISOLATES OF NEISSERIA GONORRHOEAE CARRYING THE «MOSAIC» ALLELE OF THE PENA GENE

To cite this article:

Kubanov AA, Solomka VS, Shpilevaya MV, et al. Diversity of ng-mast and mlst sequence types in Russian clinical isolates of Neisseria gonorrhoeae carrying the «mosaic» allele of the penA gene. Molecular Genetics, Microbiology and Virology. 2022;40(4):14‑21. (In Russ.)
https://doi.org/10.17116/molgen20224004114

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

GOAL

Of the study was molecular typing of current N. gonorrhoeae clinical isolates in Russia, aimed to identification of epidemiologically significant NG-MAST and MLST sequence types carrying a «mosaic» penA gene allele with reduced sensitivity to third-generation cephalosporins, which are recommended as first-line antimicrobials for gonococcal infection therapy.

MATERIAL AND METHODS

The study included 326 N. gonorrhoeae clinical isolates collected in Russia in 2018—2020. NG-MAST typing was based on sequencing of porB and tbpB genes variable regions. MLST typing was performed based on the nucleotide sequences of seven housekeeping genes: abcZ, adk, aroE, fumC, gdh, pdhC, and pgm. The penA gene structure was analyzed according to the Sanger protocol.

RESULTS

Molecular typing assigned the examined N. gonorrhoeae clinical isolates to 110 NG-MAST and 41 MLST different sequence types, respectively. The penA gene sequencing revealed a «mosaic» type XXXIV allele encoding penicillin-binding protein 2 with reduced affinity to cephalosporins in 7 (2.1%) clinical isolates. Comparison of these results showed presence of «mosaic» penA allele in clinical isolates related to STNG-MAST2212/STMLST1901 (n=1); STNG-MAST3149/STMLST1901 (n=1); STNG-MAST5622/STMLST14011 (n=1) and STNG-MAST10025/STMLST1902 (n=4) sequence types. Phylogenetic analysis has linked all named sequence types to the internationally spread, multi-resistance clone STNG-MAST1407/STMLST1901, which is a public health concern globally.

CONCLUSION

The study showed the diversity of current N. gonorrhoeae sequence types carrying the «mosaic» penA gene allele, probably as a result of novel mutational events in the porB, adk, and fumC genes taken into account by the NG-MAST and MLST protocols.

Keywords:

Neisseria gonorrhoeae
molecular typing
phylogenetic analysis

Authors:

Kubanov A.A.

State Research Center of Dermatovenerology and Cosmetology of the Ministry of Healthcare of the Russian Federation

Solomka V.S.

State Research Center of Dermatovenerology and Cosmetology

Shpilevaya M.V.

State Research Center of Dermatovenerology and Cosmetology

Verbenko D.A.

State Research Center of Dermatovenerology and Cosmetology

Deryabin D.G.

State Research Center of Dermatovenerology and Cosmetology

Kandinov I.D.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Dementieva E.I.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Gryadunov D.A.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Shaskolskiy B.L.

Center for Precision Genome Editing and Genetic Technologies for Biomedicine — Engelhardt Institute of Molecular Biology Russian Academy of Sciences

Received:

15.12.2021

Accepted:

25.04.2022

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