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Kostyaeva M.G.

Peoples’ Friendship University of Russia (RUDN University)

Kastyro I.V.

People’s Friendship University of Russia

Yunusov T.Yu.

City Clinical Hospital No. 40 of the Moscow Department of Health

Kolomin T.A.

NRC «Kurchatov Institute»-IMG

Torshin V.I.

Medical Institute of Peoples’ Friendship University of Russia

Popadyuk V.I.

Peoples’ Friendship University of Russian

Dragunova S.G.

Peoples’ Friendship University of Russia (RUDN University)

Shilin S.S.

Peoples’ Friendship University of Russia (RUDN University)

Kleyman V.K.

People’s Friendship University of Russia

Slominsky P.A.

The Institute of Molecular Genetics of National Research Centre «Kurchatov Institute»

Teplov A.Y.

Kazan State Medical University of the Ministry of Health of Russia

Protein p53 expression and dark neurons in rats hippocampus after experimental septoplasty simulation

Authors:

Kostyaeva M.G., Kastyro I.V., Yunusov T.Yu., Kolomin T.A., Torshin V.I., Popadyuk V.I., Dragunova S.G., Shilin S.S., Kleyman V.K., Slominsky P.A., Teplov A.Y.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2022;40(1): 21‑27

DOI: 10.17116/molgen20224001121

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Table of contents

PROTEIN P53 EXPRESSION AND DARK NEURONS IN RATS HIPPOCAMPUS AFTER EXPERIMENTAL SEPTOPLASTY SIMULATION
PROTEIN P53 EXPRESSION AND DARK NEURONS IN RATS HIPPOCAMPUS AFTER EXPERIMENTAL SEPTOPLASTY SIMULATION

To cite this article:

Kostyaeva MG, Kastyro IV, Yunusov TYu, et al. . Protein p53 expression and dark neurons in rats hippocampus after experimental septoplasty simulation. Molecular Genetics, Microbiology and Virology. 2022;40(1):21‑27. (In Russ.)
https://doi.org/10.17116/molgen20224001121

Подписка 2025-2 (Molecular Genetics, Microbiology and Virology)
 
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AIMS

The study evaluates the dependence of p53 protein expression on the appearance of dark neurons (DN) in the hippocampus in rats during experimental modeling of septoplasty.

MATERIALS AND METHODS

Septoplasty simulation was carried out on 15 sexually mature male Wistar rats. We studied histological sections of the hippocampus stained with Nissl toluidine blue and antibodies to the p53 protein.

RESULTS

In the CA1 subfield, the number of p53-positive neurons significantly increased on the 2nd, 4th (p<0.001) and 6th days (p<0.05). In the dynamics, the peak of the growth of p53 protein expression in the cytoplasm of CA1 and CA2 neurons fell on the 2—4th day after the operation, and on the 6th day the number of these neurons decreased (p<0.001). In the cytoplasm of CA3 neurons at all periods after surgery, an increase in the expression of the p53 protein was noted as compared to the control group. In the CA1 pyramidal layer, the number of DN decreased on the 6th day (p<0.001). In CA2, after 2 days, a minimum of DN was noted, compared with the 4th day (p<0.001). In CA3, on the 4th day, there was a peak in DN, compared with the rest of the days (p<0.001). A positive strong association was found at all periods of assessment and in all subfields of the hippocampus between an increase in the number of dark and p53-positive neurons.

CONCLUSIONS

The appearance of dark and p53-positive neurons in the hippocampal formation in rats after simulating septoplasty are typical responses of nervous tissue to stress. It is obvious that the expression of the p53 protein is associated with the basophilia of the cytoplasm of neurons, their morpho-functional state. Presumably, the p53 protein can trigger not only the activation of damaged neurons in the hippocampus, but also play a neuroprotective role. Upcoming studies should determine the role of the p53 protein in the further fate of damaged neurons in the pyramidal layer and differentiate the mechanisms of its expression.

Keywords:

septoplasty
hippocampus
dark neurons
p53
apoptosis
neurogenesis

Authors:

Kostyaeva M.G.

Peoples’ Friendship University of Russia (RUDN University)

Kastyro I.V.

People’s Friendship University of Russia

Yunusov T.Yu.

City Clinical Hospital No. 40 of the Moscow Department of Health

Kolomin T.A.

NRC «Kurchatov Institute»-IMG

Torshin V.I.

Medical Institute of Peoples’ Friendship University of Russia

Popadyuk V.I.

Peoples’ Friendship University of Russian

Dragunova S.G.

Peoples’ Friendship University of Russia (RUDN University)

Shilin S.S.

Peoples’ Friendship University of Russia (RUDN University)

Kleyman V.K.

People’s Friendship University of Russia

Slominsky P.A.

The Institute of Molecular Genetics of National Research Centre «Kurchatov Institute»

Teplov A.Y.

Kazan State Medical University of the Ministry of Health of Russia

Received:

21.08.2021

Accepted:

17.10.2021

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