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Stepanova E.A.

Moskovskiĭ oblastnoĭ nauchno-issledovatel'skiĭ klinicheskiĭ institut im. M.F. Vladimirskogo

Krutikova E.V.

Federal State Budgetary Scientific Institution «Institute of Experimental Medicine», 197376 St. Petersburg, Russia, Akad. Pavlova Street, 12, Russia

Kiseleva I.V.

Federal State Budgetary Scientific Institution «Institute of Experimental Medicine», 197376 St. Petersburg, Russia, Akad. Pavlova Street, 12, Russia;
Saint-Petersburg State University, 199034, St. Petersburg, Universitetskaya Nab., 7-9, Russia

Rudenko L.G.

Federal State Budgetary Scientific Institution «Institute of Experimental Medicine», 197376 St. Petersburg, Russia, Akad. Pavlova Street, 12, Russia

The spatial location of single amino acid substitutions in proteins of cold-adapted influenza B viruses and their impact upon cold adaptation

Authors:

Stepanova E.A., Krutikova E.V., Kiseleva I.V., Rudenko L.G.

More about the authors

Journal: Molecular Genetics, Microbiology and Virology. 2018;36(3): 13‑24

DOI: 10.17116/molgen20183603113

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Table of contents

THE SPATIAL LOCATION OF SINGLE AMINO ACID SUBSTITUTIONS IN PROTEINS OF COLD-ADAPTED INFLUENZA B VIRUSES AND THEIR IMPACT UPON COLD ADAPTATION
THE SPATIAL LOCATION OF SINGLE AMINO ACID SUBSTITUTIONS IN PROTEINS OF COLD-ADAPTED INFLUENZA B VIRUSES AND THEIR IMPACT UPON COLD ADAPTATION

To cite this article:

Stepanova EA, Krutikova EV, Kiseleva IV, Rudenko LG. The spatial location of single amino acid substitutions in proteins of cold-adapted influenza B viruses and their impact upon cold adaptation. Molecular Genetics, Microbiology and Virology. 2018;36(3):13‑24. (In Russ.)
https://doi.org/10.17116/molgen20183603113

Подписка 2026 Молекулярная генетика, микробиология и вирусология (Molecular Genetics, Microbiology and Virology)
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Abstract:

This review summarizes locations of unique amino acid substitutions in proteins of cold-adapted influenza B viruses — master donor viruses of live attenuated influenza vaccines: B/Ann Arbor/1/66ca, B/USSR/60/69, B/Leningrad/14/17/55, B/Victoria/2/63/87, and B/Vienna/1/99ca. The analysis is based on the data of the viruses’ sequences and information on the functional organization of internal proteins of influenza B virus. A description is given of the main features of the structure of internal proteins of influenza B virus and a recent understanding of the functioning of their domains. 35 substitutions in proteins of the ribonucleoprotein complex [PB1 (3), PB2 (10), PA (11), NP (11)] of cold-adapted influenza B viruses have been discussed. In total there are 44 amino acid positions which are considered to be associated with cold adaptation. Protein domains involved in protein-protein interactions are the most susceptible to changes. In the polymerase complex, the largest number of replacements was found in C-terminal domains of PA and PB2 proteins, forming the external parts of polymerase complex structure and providing interaction among subunits, with cellular factors and with the nucleoprotein (NP). Substitutions in the NP are located in the areas responsible for its oligomerization (flexible N-terminal fragment), as well as for interaction with the polymerase complex (outer surface of NP head and body domains). All the cold-adapted viruses had changes in the 7th segment of the genome, leading to amino acid substitutions in the matrix protein or BM2 proton channel. Analysis of the location of amino acid substitutions allow to assume important role of internal gene constellation in attenuation of cold-adapted viruses.

Keywords:

review
influenza B virus
cold adaptation
temperature-sensitivity
attenuation

Authors:

Stepanova E.A.

Moskovskiĭ oblastnoĭ nauchno-issledovatel'skiĭ klinicheskiĭ institut im. M.F. Vladimirskogo

Krutikova E.V.

Federal State Budgetary Scientific Institution «Institute of Experimental Medicine», 197376 St. Petersburg, Russia, Akad. Pavlova Street, 12, Russia

Kiseleva I.V.

Federal State Budgetary Scientific Institution «Institute of Experimental Medicine», 197376 St. Petersburg, Russia, Akad. Pavlova Street, 12, Russia;
Saint-Petersburg State University, 199034, St. Petersburg, Universitetskaya Nab., 7-9, Russia

Rudenko L.G.

Federal State Budgetary Scientific Institution «Institute of Experimental Medicine», 197376 St. Petersburg, Russia, Akad. Pavlova Street, 12, Russia

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