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Grigoryan D.A.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Matsvay A.D.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Chernitsov A.V.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Shipulin G.A.

Center for Strategic Planning and Management of Medical and Biological Health Risks

High-throughput sequencing: a powerful tool for pathogen detection and identification in clinical samples

Authors:

Grigoryan D.A., Matsvay A.D., Chernitsov A.V., Shipulin G.A.

More about the authors

Journal: Laboratory Service. 2024;13(4): 49‑56

DOI: 10.17116/labs20241304149

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Table of contents

HIGH-THROUGHPUT SEQUENCING: A POWERFUL TOOL FOR PATHOGEN DETECTION AND IDENTIFICATION IN CLINICAL SAMPLES
HIGH-THROUGHPUT SEQUENCING: A POWERFUL TOOL FOR PATHOGEN DETECTION AND IDENTIFICATION IN CLINICAL SAMPLES

To cite this article:

Grigoryan DA, Matsvay AD, Chernitsov AV, Shipulin GA. High-throughput sequencing: a powerful tool for pathogen detection and identification in clinical samples. Laboratory Service. 2024;13(4):49‑56. (In Russ.)
https://doi.org/10.17116/labs20241304149

 
Подписка 2025-2 (Laboratory Service)
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Recent advancements in high-throughput sequencing have significantly expanded the possibilities for diagnosing and surveilling infectious diseases. This article offers a comprehensive examination of key sequencing methodologies, emphasizing their unique characteristics and potential applications in clinical practice. Both metagenomic and targeted approaches are assessed in terms of their effectiveness, limitations, and feasibility across various diagnostic fields. We also discuss current challenges in standardization and the analysis of large genomic datasets, highlighting the urgent need for innovative bioinformatics solutions to streamline these processes. The technologies explored in this article are already transforming strategies for diagnosing infectious diseases, and their continued development may play a crucial role in the evolution of clinical diagnostic systems.

Keywords:

NGS
high-throughput sequencing
nanopore sequencing
data analysis
pathogens
clinical diagnostics
infectious diseases
metagenomic sequencing
targeted sequencing
epidemiological monitoring

Authors:

Grigoryan D.A.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Matsvay A.D.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Chernitsov A.V.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Shipulin G.A.

Center for Strategic Planning and Management of Medical and Biological Health Risks

Received:

05.11.2024

Accepted:

08.11.2024

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