Abstract Introduction. The appearance and spreading of strains of gram-negative bacteria resistant to carbapenems, leads to the ineffectiveness of antibiotic therapy of patients with severe, life-threatening infections. Of particular concern is the rapid spread of carbapenem-resistant strains of enterobacteria in hospitals. The goal of the study is to analyze the prevalence of genes of the most common carbapenemases of enterobacteria circulating in the hospital, to compare the results obtained with the level of production of carbapenemas in other hospitals of the Russian Federation. Material and methods. 769 strains of enterobacteria isolated from clinical material (blood, wound discharge, sputum, bronchoalveolar lavage, urine) were studied. Investigation of strains was conducted in three phases: the screening of Enterobacteriaceae, insensitive (resistant or with intermediate sensitivity) to carbapenems (meropenem, imipenem, ertapenem) using standard methods, the definition of karbapenemaz products from non-susceptible to carbapenems strains using the phenotypic method of carbapenem inactivation (Carbapenem inactivation method, CIM), the determination of carbapenemas types using real-time PCR metod. Results. 19% of all studied strains showed insensitivity to one of the carbapenems, 13% demonstrated the production of carbapenemases in the phenotypic method. The genes of the most common carbapenemases were found in all carbapenemase-producing strains. Among the enterobacteria, producing carbapenemases, K. pneumoniae has the largest share (87% of the total), P. mirabilis — 7%, E. coli — 4%, E. cloacae — 2%. Among carbapenemases of K. pneumoniae, 47.1% are OXA-48, 32.1% are NDM, and 18.4% are a combination of OXA-48 and NDM. One strain producing OXA-48 and VIM carbapenemases and one strain producing NDM and VIM carbapenemases were found. All 7 studied E. coli strains produce OXA-48 carbapenemase, all P. mirabilis strains (4 strains) and E. cloaceae (2 strains) produce NDM. 55% of enterobacteria, producing carbapenemases, carry genes encoding production of metallobetalactamases, among which NDM prevails (in 54 strains out of 55). Findings. The frequency of occurrence of various types of enterobacteria carbapenemases was studied. A comparative analysis of the results obtained with the data from AMRmap online resource and published works has been carried out. A set of carbapenemases of enterobacteria circulating in the hospital is typical for the territory of the Russian Federation and Moscow, but it has its own local features: they are distinguished by a high proportion of NDM carbapenemases and the combined production of OXA-48 and NDM carbapenemases.