The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Pavlova E.V.

Surgut Clinical Dermatovenerologic Dispensary, Surgut, Russia, 628403

Mihailov R.V.

Anterix, Moscow region, Russia

Kazakov A.S.

GKB #50, Moskva

Development of recombinant antibodies specific to galectin-3 by the phage display method for diagnostics and research purposes

Authors:

Pavlova E.V., Mihailov R.V., Kazakov A.S.

More about the authors

Journal: Laboratory Service. 2019;8(1): 81‑85

DOI: 10.17116/labs2019801181

Read: 1424 times

Download PDF (RU)
Contact the author
Table of contents

DEVELOPMENT OF RECOMBINANT ANTIBODIES SPECIFIC TO GALECTIN-3 BY THE PHAGE DISPLAY METHOD FOR DIAGNOSTICS AND RESEARCH PURPOSES
DEVELOPMENT OF RECOMBINANT ANTIBODIES SPECIFIC TO GALECTIN-3 BY THE PHAGE DISPLAY METHOD FOR DIAGNOSTICS AND RESEARCH PURPOSES

To cite this article:

Pavlova EV, Mihailov RV, Kazakov AS. Development of recombinant antibodies specific to galectin-3 by the phage display method for diagnostics and research purposes. Laboratory Service. 2019;8(1):81‑85. (In Russ.)
https://doi.org/10.17116/labs2019801181

Подписка 2025 Лабораторная служба (Laboratory Service)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Recommended articles:
The influence of endo­metriosis on the quality of life and reproductive function of modern woman. Russian Journal of Human Reproduction. 2024;(5):101-107
Loca­lization features of FAP-positive acti­vated stro­mal cells in fibrosis in patients with new coro­navirus infe­ction COVID-19. Russian Journal of Archive of Pathology. 2024;(6):5-14
Pathogenesis of fibrosis deve­lopment in ovarian endo­metriosis. Russian Journal of Archive of Pathology. 2025;(2):73-78
Liver invo­lvement in Gaucher disease in children: A lite­rature review and clinical obse­rvations. Russian Journal of Evidence-Based Gastroenterology. 2025;(2):78-93
Surgical correction in seco­ndary face­lift. Plastic Surgery and Aesthetic Medi­cine. 2025;(2-2):103-107
Abstract:

ABSTRACT Galectin-3 (Gal-3) is 26 kDa galactoside-binding protein which found both inside the cell and in the extracellular space. Rise of Gal-3 level in serum significantly correlated with various pathologies, such as cancer, cardiac insufficiency and fibrosis. Despite the proven prognostic significance, the widespread measurement of the level of galectin-3 in the serum of patients in clinical practice is not yet been achieved. The purpose of this study was development of recombinant human antibodies specific to galectin-3 by phage display method. As a result four antibodies to gal-3 were obtained by the phage display method. Affinity of antibodies is from 9 nM to 120 pM. The presence of the sandwich-pair in obtained antibodies allows determining the concentration of galectin-3 in the solution by the method of enzyme immunoassay (ELISA).

Keywords:

galectin-3
antibodies
phage display
malignant tumors
fibrosis

Authors:

Pavlova E.V.

Surgut Clinical Dermatovenerologic Dispensary, Surgut, Russia, 628403

Mihailov R.V.

Anterix, Moscow region, Russia

Kazakov A.S.

GKB #50, Moskva

Close metadata

References:

  1. Endo K, Kohnoe S, Tsujita E. Galectin-3 expression is a potent prognostic marker in colorectal cancer. Anticancer Research. 25(4):3117-3121. PMID: 16080575
  2. Houzelstein D, Goncalves IR, Fadden AJ, Sidhu SS, Cooper DN, Drickamer K, Leffler H, Poirier. Phylogenetic analysis of the vertebrate galectin family. Mol Biol Evol. 2004 Jul;21(7):1177-1187. https://doi.org/10.1093/molbev/msh082
  3. Dumic J, Dabelic S, Flogel M. Galectin-3: an open-ended story. Biochemica and biophysica Acta. 2006 Apr;1760(4):616-635. https://doi.org/10.1016/j.bbagen.2005.12.020
  4. Shimura T, Shibata M, Gonda K, Nakajima T, Chida S, Noda M, Suzuki S, Nakamura I, Ohki S, Takenoshita S. Association between circulating galectin-3 levels and the immunological, inflammatory and nutritional parameters in patients with colorectal cancer. Biomed Rep. 2016 Aug;5(2):203-207. https://doi.org/10.3892/br.2016.696
  5. Traber P, Chou H, Zomer E, Hong F, Klyosov K, Frel MI, Friedman SL. Regression of fibrosis and reversal of cirrhosis in rats by galectin inhibitors in thioacetamide-induced liver disease. PLOS One. 2013 Oct 9;8(10):e75361. https://doi.org/10.1371/journal.pone.0075361
  6. Kramer F. Galectin-3: clinical utility and prognostic value in patients with heart failure. Research reports in Clinical Cardiology. 2013;4:13-22. https://doi.org/10.2147/RRCC.S28562
  7. Danguy A, Camby I, Kiss R. Galectins and cancer. Biochim Biophys Acta. 2002 (1572);285-293. PMID: 12223276
  8. MacKinnon AC, Farnworth SL, Hodkinson PS, Henderson NC, Atkinson KM, Leffler H, Nilsson UJ, Haslett C, Forbes SJ, Sethi T. Regulation of alternative macrophage activation by galectin-3. J Immunol. 2008 Feb 15;180(4):2650-2658. https://doi.org/10.4049/jimmunol.180.4.2650
  9. Connie Arthur M, Cataldi Rodrigues L, Dias Baruffi M, Harold Sullivan C, Heimburg-Molinaro J, Dave Smith F, Richard Cummings D, Sean Stowell R. Examining Galectin Binding Specificity Using Glycan Microarrays. Methods Mol Biol. 2015;1207:115-131. https://doi.org/10.1007/978-1-4939-1396-1_8
  10. Barbas CFI, Burton DR, Silverman GJ. Phage Display: A Laboratory Manual. New York, NY: Cold Spring Harbor. 2001.
  11. Delafosse L, Xu P, Durocher Y. Comparative study of polyethylenimines for transient gene expression in mammalian HEK293 and CHO cells. J Biotechnol. 2016 Jun 10;227:103-111. https://doi.org/10.1016/j.jbiotec.2016.04.028
  12. Chang L, Li J, Wang L. Immuno-PCR: An Ultrasensitive Immunoassay for Biomolecular Detection. Analytica Chimica Acta. 2016;910:12-24. https://doi.org/10.1016/j.aca.2015.12.039
  13. Orlov AV, Znoyko SL, Cherkasov VR, Nikitin MP, Nikitin PI. Multiplex Biosensing Based on Highly Sensitive Magnetic Nanolabel Quantification: Rapid Detection of Botulinum Neurotoxins A, B, and E in Liquids. Anal Chem. 2016;88(21):10419-10426. https://doi.org/10.1021/acs.analchem.6b02066
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.