Claudin-1 expression in epithelial skin tumor cells

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ACTUALIZATION

Claudin-1 is a protein that is an integral part of the tight junctions of epidermal cells. Until the end, its role in the process of carcinogenesis is not clear. In colorectal and breast cancer cells, the appearance of pathological expression of claudin-1 or its complete loss was noted, which is associated. The appearance of a clear expression in the cells of the invasive front of cervical cancer correlated with the appearance of regional metastases. Decreased expression of claudin-1 was observed in squamous cell carcinoma of the skin and actinic keratosis compared with normal skin, complete disappearance of expression was observed in squamous cell carcinoma of the oral mucosa compared with leukoplakia. The role of claudin-1 in the growth of benign and malignant skin tumors is not fully understood.

OBJECTIVE

Study of the expression of claudin-1 in epithelial skin tumor cells.

MATERIALS AND METHODS

Immunohistochemical study according to the standard protocol with antibodies to claudin-1 from biopsy material of 51 epithelial skin tumors.

RESULTS

Half of the cells of squamous and basal cell carcinomas of the skin abnormally expressed claudin-1: in the former, the absence of immunoreactivity was significantly more frequent, in the latter, expression in the cytoplasm; in the cell complexes, a chaotic distribution of cells with different types of immunoreactivity was observed. In the cells of actinic keratosis, seborrheic keratosis, and interning follicular keratosis, membrane expression of claudin-1 was statistically significantly predominant, and the zoning of the distribution of immunoreactivity was similar to the normal epidermis.

CONCLUSION

In cells of malignant epithelial skin tumors, pathological expression of claudin-1 prevailed: expression in the cytoplasm in basal cell carcinoma and lack of immunoreactivity in squamous cell carcinoma. In the cells of AK, SK, and IPK, predominantly membrane expression of claudin-1 and a clear zoning of the distribution of cells expressing the marker were observed.

Keywords:

claudin-1

membrane expression

cytoplasmic expression

squamous cell carcinoma

basal cell carcinoma

actinic keratosis

seborrheic keratosis

Authors:

Khlebnikova A.N.

M.F. Vladimirsky Moscow Regional Research Clinical Institute

Scopus AuthorID: 6507373964
ORCID: 0000-0003-4400-5631

Sedova T.G.

E.A. Vagner Perm State Medical University

ORCID: 0000-0002-2660-0536

Gurevich L.E.

M.F. Vladimirsky Moscow Regional Research and Clinical Institute

Selezneva E.V.

M.F. Vladimirsky Moscow Regional Research and Clinical Institute

ORCID: 0000-0002-6181-9031

Molochkov A.V.

M.F. Vladimirsky Moscow Regional Research Clinical Institute;
Peoples’ Friendship University of Russia

Received:

16.05.2020

Accepted:

20.11.2020

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