A new method is proposed for the assessment of effectiveness of the treatment of trophic ulcers using a laser for videoanalysis of two-dimensional images of a given area at the tissue surface. The potential of fluorescent diagnostics of pathological changes in soft tissues of the lower extremities was evaluated in 27 patients (19 men and 8 women aged 35 to 60 years) undergoing treatment for trophic lesions with concomitant chronic venous diseases (CVD). Trophic ulcers (CEAP class C6) occurred in 21 patients and epithelialized ulcers in the remaining six. Primary and post-thrombotic varicosis was diagnosed in 19 and 8 patients respectively. Results of the study show that measurement of fluorescent radiation intensity in affected tissues is a valuable non-invasive diagnostic method allowing for highly reliable evaluation of treatment efficiency and the choice of strategy for its continuation.