Expression of terminal deoxynucleotidyl transferase in the pharyngeal and palatine tonsils in local infectious and inflammatory diseases of the upper respiratory tract and pharynx in childhood

Bykova V.P.

doi:https://doi.org/10.17116/patol20218306127

Bykova V.P.

Scientific and Clinical Center of Otorhinolaryngology

Expression of terminal deoxynucleotidyl transferase in the pharyngeal and palatine tonsils in local infectious and inflammatory diseases of the upper respiratory tract and pharynx in childhood

Authors:

Bykova V.P.

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Journal: Russian Journal of Archive of Pathology. 2021;83(6): 27‑34

DOI: 10.17116/patol20218306127

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To cite this article:

Bykova VP. Expression of terminal deoxynucleotidyl transferase in the pharyngeal and palatine tonsils in local infectious and inflammatory diseases of the upper respiratory tract and pharynx in childhood. Russian Journal of Archive of Pathology. 2021;83(6):27‑34. (In Russ.)
https://doi.org/10.17116/patol20218306127

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)

Использование инфографики авторами научных работ

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Abstract:

Along with central immune organs, the peripheral lymphoepithelial organs of the pharynx are actively involved in protecting the body from infections. Adaptive, or induced, immunity occurs during the postnatal ontogenesis of immunocompetent lymphocytes, which includes the secondary somatic recombination of the V genes with the participation of recombination-activating gene (RAG) and terminal deoxynucleotidyl transferase (Tdt) proteins. This publication discusses the results of detection of Tdt-positive cells in the pharyngeal and palatine tonsils of children of different ages, who had been operated on for adenoid vegetations and chronic tonsillitis. Attention is drawn to the localization of Tdt⁺ cells, the level of Tdt expression, an attempt to clarify the phenotype, destination, and place in the diagnostic arrays of functional markers when an adaptive immunity is generated in children.

Keywords:

terminal deoxynucleotidyl transferase

somatic recombination

immune response

adenoids

chronic tonsilitis

Authors:

Bykova V.P.

Scientific and Clinical Center of Otorhinolaryngology

Received:

07.06.2021

Accepted:

17.09.2021

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References:

Roitt I, Brostoff J, Male D. Immunology. London: Mosby.
Kriglstein Warrren. Where is the self and where is the non-self, or the mazes of autotolerance. New medicine of the millenium. Immunology. 2009;3:18-27. (In Russ.).
Babaeva AG. Regeneratsiya I systema immunogeneza. M.: Meditsina; 1985. (In Russ.).
Yushkov BG, Klimin VG, Tkachenko AE, Dugina EA. Strukturnyj gomeostaz. M.: Kommentariy; 2019. (In Russ.).
Калюжин О.В. Возможности использования пробиотиков для укрепления противоинфекционной защиты в свете иммуногомеостатической роли микробиоты. Эффективная фармакотерапия. Аллергология и иммунология. 2013;27(2):12-25.
Kalyuzhin OV. Vozmozhnosti ispolzovaniya probiotikov dlya ukrepleniya protivoinfektsionnoy zashchity v svete immunogomeostaticheskoy roli mikrobioty. Effektivnaya pharmakoterapiya. Allergologiya i immunologiya. 2013;27(2):12-25.(In Russ.).
Hertz M, Nemazee D. BCR ligation induces receptor editing in IgM+/IgD– bone marrow B cells in vitro. J Immunology. 1997;6:429.
Han S, Zheng B, Schatz DG, Spandopoulou E, Kelsoe G. Neoteny in lymphocytes: Rag1 and Rag2 expression in germinal center B cells. Science. 1996;247:2094.
Oettinger MA, Schatz DG, Gorka C, Baltimore D. RAG-1 and RAG-2, adjacent genes that sinergistically activate V(D)J recombination. Science. 1990;248:1517.
Gellert M. V(D)J recombination: RAG proteins, repair factors and regulation. Annu Rev Biochem. 2002;71:101-132.
Swanson PC. The bounty of RAGs: recombination signal complexes and reaction outcomes. Immunol Rev. 2004;200:90-114.
Loc’h J, Delarue M. Terminal deoxynucleotidyltransferase: the story of an untemplated DNA capable of DNA bridging and templated synthesis across strands. Current Opinion in Structural Biology. 2018;53:22-31. https://doi.org/10.1016/j.sbi.2018.03.019
Yarilin AA. Immunologiya: uchebnik. M.: GEOTAR-Media; 2010. (In Russ.).
Janeway CA Jr, Medzhitov R. Innate immune recognition. Ann Rev Immunol. 2002;20:197-216.
Pasare C, Medzhitov R. Toll-like receptors: linking innate and adaptivde immunity. Microb Infect. 2004;6(15):1382-1387.
Cattoretti G, Parravicini C, Bonati A, at al. Terminal deoxynucleotidyl transferase-posinive B cell precursors in fetal lymph nodes and extrahemopoietic tissues. Eur J Immunol. 1989;19:493-500.
Girschick HJ, Crammer C, Nanki T, Mayo M, Lipsky PE. RAG-1 and RAG-2 expression by B cell subsets from human tonsil and peripheral blood. J Immunol. 2001;166:377.
Bollum FJ. Calf thymus polymarase. J Biol Chemistry. 1960;235(8): 2399-2403. https://doi.org/10.1016/S0021-9258(18)64634-4
Meru N, Jung A, Baumann Ir, Niedobitek G. Expression of the recombination-activating genes in extrafollicular lymphocytes but no apparent reinduction in germinal center reactions in human tonsil. Blood. 2002;99(1-2):531-537.
Bykova VP, Belavina PI, Ryasanskaya AG, Yunusov AS. The state of the pharyngeal and palatine tonsils in children under the modern conditions of infectious and inflammatory diseases of the upper respiratory tract and the pharynx including the application of the immunotropic medications. Russian Rhinology. 2018;26(2):3-14. (In Russ.). https://doi.org/10.17116/rosrino20182623
Kalinin DV, Bykova VP. Gistoarhitectonica glotochnoi mindaliny v vosrastnom aspecte. Morphometricheskoe I immunogistohimicheskoe issledovanie. Arkhiv patologii. 2011;73(1):14-19. (In Russ.).
Yarilin AA, Donetskova AD. T-kletki — nedavnie emigranty is timusa. Immunologiya. 2012;33(6):326-334. (In Russ.).
Hillion S, Saraux A, Youinou P, Jamin Ch. Expression of RAGs in peripheral B cells outside germinal centers is associated with expression CD5. J Immunol. 2005;174:5553-5561.
Zhang Y, Shi M, Wen Q, Luo W, Yang Z, Zhou M, Ma L. Antigenic stimulation induces recombination atnvating gene 1 and terminal deoxynucleotidyl transferase expression in a murin T-cell hybridoma. Cell immunol. 2012;274(1-2):19-25. https://doi.org/10.1016/j.cellimm.2012.02.008
Tracy CK, Schlissel MS. Mechanisms controlling expression of the RAG locus during lymphocyte development. Curr Opin Immunol. 2009;21(2):173-178. https://doi.org/10.1016/j.coi.2009.03.008
Brandtzaeg P, Pabst R. Let’s go mucosal: communication on slippery ground. Opinion. Tin Immunology. 2004;25(11):570-577.