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Sokologorskiy S.V.

I.M. Sechenov First Moscow State Medical University, 119991, Moscow, Russia ,

Glycocalyx — birth of a new clinical paradigm

Authors:

Sokologorskiy S.V.

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Journal: Russian Journal of Anesthesiology and Reanimatology. 2018;(4): 22‑29

DOI: 10.17116/anaesthesiology201804122

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GLYCOCALYX — BIRTH OF A NEW CLINICAL PARADIGM
GLYCOCALYX — BIRTH OF A NEW CLINICAL PARADIGM

To cite this article:

Sokologorskiy SV. Glycocalyx — birth of a new clinical paradigm. Russian Journal of Anesthesiology and Reanimatology. 2018;(4):22‑29. (In Russ.)
https://doi.org/10.17116/anaesthesiology201804122

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Abstract:

Endothelial dysfunction underlies many diseases and pathological processes. It was believed for a long time that these disorders are a consequence of direct damage and malfunction of the endothelial cell itself. A huge number of studies showing that the true cause of those dysfunctions is the destruction of glycocalyx — a thin molecular structure of the proteoglycans, glycoproteins and glycosaminglycans located on the endothelial surface, has appeared recently. The structure, function, and destruction of the glycocalyx are reviewed.

Keywords:

glycocalyx
endothelium
dysfunction

Authors:

Sokologorskiy S.V.

I.M. Sechenov First Moscow State Medical University, 119991, Moscow, Russia ,

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References:

  1. Danielli JF. Capillary permeability and oedema in the perfused frog. J Physiol. 1940;98:109-129. https://doi.org/10.1113/jphysiol.1940.sp003837
  2. Farquhar MGGE. Palade, Segregation of ferritin in glomerular protein absorption droplets. J Biophys Biochem Cytol. 1960;7:297-304.
  3. van den Berg BM, Vink HE, Spaan JA. The Endothelial Glycocalyx Protects Against Myocardial Edema. Circ Res. 2003;92:592-594.
  4. van den Berg BM, Nieuwdorp M, Stroes ES, Vink H. Glycocalyx and endothelial (dys) function: from mice to men. Pharmacol Rep. 2006;58:Suppl:75-80.
  5. Reitsma S, Slaaf DW, Vink H, van Zandvoort MAMJ, oude Egbrink MGA. The endothelial glycocalyx: composition, functions, and visualization. Pflugers Archiv. 2007;454(3):345-359. https://doi.org/10.1007/s00424-007-0212-8
  6. Pries AR, Secomb TW, Gaehtgens P. The endothelial surface layer. Pflügers Arch Eur J Physiol. 2000;440:653. https://doi.org/10.1007/s004240000307
  7. Luft JH. Fine structures of capillary and endocapillary layer as revealed by ruthenium red. Fed Proc. 1966;25(6):1773-1783.
  8. Bennett HS. Morphological Aspects of Extracellular Polysaccharides. Journal of Histochemistry & Cytochemistry. 1963;11(1):14-23.
  9. Desjardins C, Duling BR. Heparinase treatment suggests a role for the endothelial cell glycocalyx in regulation of capillary hematocrit. Am J Physiol 1990;258(3 pt2):H647-H654.
  10. Klitzman B, Duling BR. Microvascular hematocrit and red cell flow in resting and contracting striated muscle. Am J Physiol. 1979;237:H481-H490.
  11. Becker BF, Chappell D, Jacob M. Endothelial glycocalyx and coronary vascular permeability: the fringe benefit. Basic Res Cardiol. 2010;105:687. https://doi.org/10.1007/s00395-010-0118-z
  12. Rehm M, Zahler S, Lotsch M, et al. Endothelial glycocalyx as an additional barrier determining extravasation of 6% hydroxyethyl starch or 5% albumin solutions in the coronary vascular bed. Anesthesiology. 2004;100:1211-1223.
  13. Gouverneur M, Spaan JA, Pannekoek H, Fontijn RD, Vink H. Fluid shear stress stimulates incorporation of hyaluronan into endothelial cell glycocalyx. Am J Physiol Heart Circ Physiol. 2006;290(1):H458-H552.
  14. Lipowsky HH. Microvascular rheology and hemodynamics. Microcirculation 2005;12:5-15.
  15. Becker BF, Chappell D, Bruegger D, Annecke T, Jacob M. Therapeutic strategies targeting the endothelial glycocalyx: acute deficits, but great potential. Cardiovas Res. 2010;87:300-310. https://doi.org/10.1093/cvr/cvq137
  16. Nieuwdorp M, Meuwese MC, Mooij HL, et al. Measuring endothelial glycocalyx dimensions in humans: a potential novel tool to monitor vascular vulnerability. J Appl Physiol. 2008;104:845-852.
  17. Alberts B, Johnson A, Lewis J, Raff M, Roberts K,Walter P. Molecular biology of the cell: Reference edition. NY: Garland Science; 2007. ISBN 13:9780815341116
  18. Ausprunk DH, Boudreau CL, Nelson DA. Proteoglycans in the microvasculature. I. Histochemical localization in microvessels of the rabbit eye. Am J Pathol. 1981;103(3):353-566.
  19. Culty M, et al. The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins. J Cell Biol. 1990;111(6 Pt 1):2765-2774.
  20. Jacob M, Bruegger D, Rehm M, et al. The endothelial glycocalyx affords compatibility of Starling’s principle and high cardiac interstitial albumin levels. Cardiovasc Res. 2007;73(3):575-586. https://doi.org/10.1016/j.cardiores.2006.11.021
  21. Levick JR. Capillary filtration-absorption balance reconsidered in light of dynamic extravascular factors. Exp Physiol. 1991;76(6):825-857.
  22. Ait-Oufella H, Maury E, Lehoux S, Guidet B, Offenstadt G. The endothelium: physiological functions and role in microcirculatory failure during severe sepsis. Intensive Care Med. 2010;36(8):1286-1298. https://doi.org/10.1007/s00134-010-1893-6
  23. Jacob M, Rehm M, Loetsch M, et al. The endothelial glycocalyx prefers albumin for evoking shear stress-induced, nitric oxide-mediated coronary dilatation. J Vasc Res. 2007;44:435-443. https://doi.org/10.1159/000104871
  24. Florian JA, Kosky JR, Ainslie K, Pang Z, Dull RO, Tarbell JM. Heparan sulfate proteoglycan is a mechanosensor on endothelial cells. Circ Res. 2003;93:136-142.
  25. Zhang F, Zhang Z, Lin X, et al. Compositional analysis of heparin/heparan sulfate interacting with fibroblast growth factor3 receptor complexes. Biochemistry. 2009;48:8379-8386.
  26. Chappell D, Jacob M, Hofmann-Kiefer K, et al. Antithrombin reduces shedding of the endothelial glycocalyx following ischaemia/reperfusion. Cardiovasc Res. 2009;83(2):388-396. https://doi.org/10.1093/cvr/cvp097
  27. Gao L, Lipowsky HH. Composition of the endothelial glycocalyx and its relation to its thickness and diffusion of small solutes. Microvasc Res. 2010;80:394-401.
  28. Rehm M, Bruegger D, Christ F, et al. Shedding of the endothelial glycocalyx in patients undergoing major vascular surgery with global and regional ischemia. Circulation. 2007;116:1896-1906.
  29. Hofmann-Kiefer KF, Kemming GI, Chappell D, et al. Serum heparan sulfate levels are elevated in endotoxemia. Eur J Med Res. 2009;14:526-531.
  30. Nieuwdorp M, Mooij HL, Kroon J, et al. Endothelial glycocalyx damage coincides with microalbuminuria in type 1 diabetes. Diabetes. 2006;55:1127-1132.
  31. Karangelis D, Asimakopoulou A, Kanakis I, et al. Monitoring serum chondroitin sulfate levels in patients submitted to coronary artery bypass surgery. Biomed Chromatogr. 2010;25:748-750.
  32. Chelazzi C, Villa G, Mancinelli P et al. Glycocalyx and sepsis-induced alterations in vascular permeability. Critical Care. 2015;19:26. https://doi.org/10.1186/s13054-015-0741-z
  33. Steppan J, Hofer S, Funke B, et al. Sepsis and major abdominal surgery lead to flaking of the endothelial glycocalix. J Surg Res. 2011;165:136-141.
  34. Johansson PI, Stensballe J, Rasmussen LS, Ostrowski SR. A high admission syndecan-1 level, a marker of endothelial glycocalyx degradation, is associated with inflammation, protein C depletion, fibrinolysis, and increased mortality in trauma patients. Ann Surg 2011;254:194-200.
  35. Devaraj S, Yun JM, Adamson G, Galvez J, Jialal I. C-reactive protein impairs the endothelial glycocalyx resulting in endothelial dysfunction. Cardiovasc Res. 2009;84:479-484.
  36. Platts SH, Duling BR. Adenosine A3 receptor activation modulates the capillary endothelial glycocalyx. Circ Res. 2004;94:77-82.
  37. VanTeeffelen JW, Brands J, Vink H. Agonist-induced impairment of glycocalyx exclusion properties: contribution to coronary effects of adenosine. Cardiovasc Res. 2010;87:311-319.
  38. Chappell D, Hofmann-Kiefer K, Jacob M, et al. TNF-alpha induced shedding of the endothelial glycocalyx is prevented by hydrocortisone and antithrombin. Basic Res Cardiol. 2009;104:78-89.
  39. VanTeeffelen JW, Constantinescu AA, Brands J, Spaan JA, Vink H. Bradykinin- and sodium nitroprusside-induced increases in capillary tube haematocrit in mouse cremaster muscle are associated with impaired glycocalyx barrier properties. J Physiol. 2008;586:3207-3218.
  40. Annecke T, Fischer J, Hartmann H, et al. Shedding of the coronary endothelial glycocalyx: effects of hypoxia/reoxygenation vs ischaemia/reperfusion. Br J Anaesth. 2011;107:679-686.
  41. Yarustovsky MB, Abramyan MV, Krotenko NP, Popov DA, Plyusch MG, Nazarova EI, Gordeev SL. Experience of use of endotoxin selective adsorption in patients with heavy sepsis after open-heart surgery. Anesteziologiya i reanimatologiya. 2014;3:39-46. (In Russ.)
  42. Nieuwdorp M, van Haeften TW, Gouverneur MC, et al. Loss of endothelial glycocalyx during acute hyperglycemia coincides with endothelial dysfunction and coagulation activation in vivo. Diabetes. 2006;55:480-486.
  43. Nelson A, Berkestedt I, Schmidtchen A, Ljunggren L, Bodelsson M. Increased levels of glycosaminoglycans during septic shock: relation to mortality and the antibacterial actions of plasma. Shock. 2008;30:623-627.
  44. Yaroshetskiy AI, Vasilieva SO, Rezepov NA, Lapshina IYu, Gelfand BR. Indirect calorimetry for evaluation of glucose and lipid metabolism in surgical patients on total parenteral nutrition: a pilot study. Vestnik intensivnoj terapii. 2016;4:12-18. (In Russ.)
  45. van den Berg BM, Spaan JA, Rolf TM, Vink H. Atherogenic region and diet diminish glycocalyx dimension and increase intima-to-media ratios at murine carotid artery bifurcation. Am J Physiol Heart Circ Physiol. 2006;290(2): H915-H920.
  46. Vink H, Constantinescu AA, Spaan JA. Oxidized lipoproteins degrade the endothelial surface layer: implications for platelet-endothelial cell adhesion. Circulation. 2000;101:1500-1502.
  47. Berg S, Engman A, Hesselvik JF, Laurent TC. Crystalloid infusion increases plasma hyaluronan. Crit Care Med. 1994;22(10):1563-1567. https://doi.org/10.1097/00003246-199422100-00010
  48. Rehm M, Haller M, Orth V, Kreimeier U, Jacob M, Dressel H, et al. Changes in blood volume and hematocrit during acute preoperative volume loading with 5% albumin or 6% hetastarch solutions in patients before radical hysterectomy. Anesthesiology. 2001;95:849-856.
  49. Moroz VV, Ryzhkov IA. Acute Blood Loss: Regional Blood Flow and Microcirculation (Review, Part I). Obshchaya reanimatologiya. 2016;12(2):66-89. (In Russ.) https://doi.org/10.15360/1813-9779-2016-2-56-65
  50. Boer C, Koning NJ, Van Teeffelen J, Vonk AB, Vink H. Changes in microcirculatory perfusion during cardiac surgery are paralleled by alterations in glycocalyx integrity. Crit Care. 2013;17(Suppl 2):212. https://doi.org/10.1186/cc12150
  51. Bruegger D, Jacob M, Rehm M, Loetsch M, Welsch U, Conzen P, et al. Atrial natriuretic peptide induces shedding of endothelial glycocalyx in coronary vascular bed of guinea pig hearts. Am J Physiol Heart Circ Physiol. 2005;289(5):H1993-H1999.
  52. Flameng W, Borgers M, Van Der Vusse G J. Cardioprotective effects of lidoflazine in extensive aorta-coronary bypass grafting. J Thorac Cardiovasc Surg. 1983;85:758-768.
  53. Obeidat M, Ballermann B.J. Glomerular endothelium: a porous sieve and formidable barrier. Exp Cell Res. 2012;318:964-972. https://doi.org/10.1016/j.yexcr.2012.02.032
  54. Jeansson M, Haraldsson B. Morphological and functional evidence for an important role of the endothelial cell glycocalyx in the glomerular barrier. Am J Physiol Renal Physiol. 2006;290(1):F111-F116. https://doi.org/10.1152/ajprenal.00173.2005
  55. Burlev AlV, Burlev VA, Ilyasova NA, Shifman EM. Oxidative stress in perioperative period of cesarean section Anesteziologiya i reanimatologiya. 2014;59(6):24. (In Russ.)
  56. Chappell D, Jacob M, Hofmann-Kiefer K, et al. Hydrocortisone preserves the vascular barrier by protecting the endothelial glycocalyx. Anesthesiology. 2007;107:776-784.
  57. Hippenstiel S, Krüll M, Ikemann A, Risau W, Clauss M, Suttorp N. VEGF induces hyperpermeability by a direct action on endothelial cells. Am J Physiol. 1998;274:L678-L684.
  58. Sarti A, De Gaudio AR, Messineo A, Cuttini M, Ventura A. Glomerular permeability after surgical trauma in children: relationship between microalbuminuria and surgical stress score. Crit Care Med. 2001;29:1626-1629.
  59. Thorevska N, Sabahi R, Upadya A, Manthous C, Amoateng-Adjepong Y. Microalbuminuria in critically ill medical patients: prevalence, predictors, and prognostic significance. Crit Care Med. 2003;31:1075-1081.
  60. Gosling P, Brudney S, McGrath L, Riseboro S, Manji M. Mortality prediction at admission to intensive care: a comparison of microalbuminuria with acute physiology scores after 24 hours. Crit Care Med. 2003;31:98-103.
  61. Pallister I, Dent C, Wise CC, Alpar EK, Gosling P. Early post-traumatic acute respiratory distress syndrome and albumin excretion rate: a prospective evaluation of a ‘point-of care’ predictive test. Injury. 2001;32(3):177-181. https://doi.org/10.1016/S0020-1383(00)00149-2
  62. Honarmand A, Safavi M, Baghery K, Momayezi A. The association of microalbuminuria and duration of mechanical ventilation in critically ill trauma patients. Ulus Travma Acil Cerrahi Derg. 2009;15:12-18.
  63. Basu S, Bhattacharya M, Chatterjee TK, Chaudhuri S, Todi SK, Majumdar A. Microalbuminuria: a novel biomarker of sepsis. Indian J Crit Care Med. 2010;14:22-28.
  64. Rinaldi S, Adembri C, Grechi S, De Gaudio AR. Low-dose hydrocortisone during severe sepsis: effects on microalbuminuria. Crit Care Med. 2006;34:2334-2339.
  65. Haywood-Watson RJ, Holcomb JB, Gonzalez EA, Peng Z, Pati S, Park PW, et al. Modulation of Syndecan-1 Shedding after Hemorrhagic Shock and Resuscitation. PLoS ONE 2011;6(8):e23530. https://doi.org/10.1371/journal.pone.0023530
  66. Sarrazin S, Lyon M, Deakin JA, Guerrini M, Lassalle P, Delehedde M, et al. Characterization and binding activity of the chondroitin/dermatan sulfate chain from Endocan, a soluble endothelial proteoglycan. Glycobiology. 2010;20:1380-1388.
  67. Mikkelsen ME, Shah CV, Scherpereel A, Lanken PN, Lassalle P, Bellamy SL, et al. Lower serum endocan levels are associated with the development of acute lung injury after major trauma. J Crit Care. 2012;27(5):522.e11-e17. https://doi.org/10.1016/j.jcrc.2011.07.077
  68. Goetz R., Mohammadi M. Exploring mechanisms of FGF signalling through the lens of structural biology. Nat Rev Mol Cell Biol. 2013;14(3):166-180. https://doi.org/10.1038/nrm3528
  69. Jiang D, Liang J, Fan J, Yu S, Chen S, Luo Y, et al. Regulation of lung injury and repair by Toll-like receptors and hyaluronan. Nat Med. 2005;11(11):1173-1179.
  70. Schmidt EP, Li G, Li L, Fu L, Yang Y, Katherine H, et al. The circulating glycosaminoglycan signature of respiratory failure in critically ill adults. J Biol Chem. 2014;289(12):8194-8202.
  71. Kranidioti H, Orfanos SE, Vaki I, Kotanidou A, Raftogiannis M, Dimopoulou I, et al. Angiopoietin-2 is increased in septic shock: evidence for the existence of a circulating factor stimulating its release from human monocytes. Immunol Lett. 2009;125:65-71.
  72. Xing K, Murthy S, Liles WC, Singh JM. Clinical utility of biomarkers of endothelial activation in sepsis — a systematic review. Crit Care. 2012;16:R7.
  73. Siner JM, Bhandari V, Engle KM, Elias JA, Siegel MD. Elevated serum angiopoietin 2 levels are associated with increased mortality in sepsis. Shock. 2009;31:348-353.
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