Blood-brain barrier permeability in healthy rats and rats with experimental C6 glioma after fractionated radiotherapy of the brain

Iusubalieva G.M.; Levinskiy A.B.; Zorkina Ia.A.; Baklaushev V.P.; Goriaĭnov S.A.; Pavlova G.V.; Mel’nikov P.A.; Gorlachev G.E.; Golanov A.V.; Potapov A.A.; Chekhonin V.P.

doi:https://doi.org/10.17116/neiro201579315-26

Iusubalieva G.M.

Otdel fundamental'noĭ i prikladnoĭ neĭrobiologii FGBU "Gosudarstvennyĭ nauchnyĭ tsentr sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo";
Kafedra meditsinskikh nanobiotekhnologiĭ Rossiĭskogo gosudarstvennogo meditsinskogo universiteta im. N.I. Pirogova

Levinskiy A.B.

N.I. Pirogov Russian State Medical University, Department of Medical Nano-biotechnology

Zorkina Ia.A.

Otdel fundamental'noĭ i prikladnoĭ neĭrobiologii FGBU "Gosudarstvennyĭ nauchnyĭ tsentr sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo"

Baklaushev V.P.

Goriaĭnov S.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Pavlova G.V.

FGBU "Institut biologii gena" RAN

SPIN RSCI: 4633-8339
Scopus AuthorID: 7005650683
ORCID: 0000-0002-6885-6601

Mel’nikov P.A.

N.I. Pirogov Russian State Medical University, Department of Medical Nano-biotechnology

Gorlachev G.E.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Golanov A.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

ORCID: 0000-0002-0976-4547

Potapov A.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Chekhonin V.P.

Blood-brain barrier permeability in healthy rats and rats with experimental C6 glioma after fractionated radiotherapy of the brain

Authors:

Iusubalieva G.M., Levinskiy A.B., Zorkina Ia.A., Baklaushev V.P., Goriaĭnov S.A., Pavlova G.V., Mel’nikov P.A., Gorlachev G.E., Golanov A.V., Potapov A.A., Chekhonin V.P.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2015;79(3): 15‑26

DOI: 10.17116/neiro201579315-26

Read: 2195 times

To cite this article:

Iusubalieva GM, Levinskiy AB, Zorkina IaA, et al. Blood-brain barrier permeability in healthy rats and rats with experimental C6 glioma after fractionated radiotherapy of the brain. Burdenko's Journal of Neurosurgery. 2015;79(3):15‑26. (In Russ., In Engl.)
https://doi.org/10.17116/neiro201579315-26

Подписка 2025 Журнал «Вопросы нейрохирургии» имени Н.Н. Бурденко (Burdenko's Journal of Neurosurgery)

Использование инфографики авторами научных работ

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Abstract:

Brain tumors are accompanied by blood-brain barrier (BBB) dysfunction. However, targeted drug delivery still remains difficult to achieve. Objective. To evaluate the effect of fractionated radiotherapy on permeability of the blood-brain barrier in healthy rats and rats with C6 glioma in vivo. Material and methods. An increase in BBB permeability in C6 glioma was assessed by dynamic MRI monitoring (glioma size before and after radiation therapy in combination with immunotherapy, n=30) and confocal microscopy (fluorescence imaging of tumor invasion boundaries in a dose-dependent experiment for the amount of injected antibodies). In healthy rats, BBB permeability to macromolecular substances (MMS) was assessed by ELISA (n=23, 192 plasma samples) and confocal microscopy (n=7). Results. It was shown that BBB permeability to biological macromolecules in blood-brain and brain-blood directions was increased after fractionated radiotherapy. Conclusion. Drug delivery to the brain can be improved using therapeutic doses of radiation treatment that affects the BBB and minimizes the risk of serious side effects that are often associated with the drug dose.

Keywords:

glioma

blood-brain barrier in norm and pathology

fractionated radiotherapy

Authors:

Iusubalieva G.M.

Levinskiy A.B.

N.I. Pirogov Russian State Medical University, Department of Medical Nano-biotechnology

Zorkina Ia.A.

Otdel fundamental'noĭ i prikladnoĭ neĭrobiologii FGBU "Gosudarstvennyĭ nauchnyĭ tsentr sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo"

Baklaushev V.P.

Goriaĭnov S.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Pavlova G.V.

FGBU "Institut biologii gena" RAN

SPIN RSCI: 4633-8339
Scopus AuthorID: 7005650683
ORCID: 0000-0002-6885-6601

Mel’nikov P.A.

N.I. Pirogov Russian State Medical University, Department of Medical Nano-biotechnology

Gorlachev G.E.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

Golanov A.V.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN, Moskva

ORCID: 0000-0002-0976-4547

Potapov A.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Chekhonin V.P.

Close metadata

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