Human glioma malignancy grade and migratory capacity depending on expression of GDNF isoforms in vitro

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OBJECTIVE

To assess the expression of mRNA splice variants of GDNF in glioma cell cultures with various malignancy grades (I—IV) and degrees of migration.

MATERIAL AND METHODS

In this study, αGDNF and βGDNF expression was analyzed in 15 human glioma cell cultures using Southern blot hybridization of GDNF cDNA and reverse transcription with PCR to amplify splice variants of GDNF mRNA.

RESULTS

The highest expression of αGDNF and βGDNF isoforms was observed in cell cultures of human gliomas with extensive migratory activity. Low βGDNF expression without αGDNF expression is typical only for gliomas with low migratory activity. In addition, we found additional patterns of mRNA expression that have not been previously described.

CONCLUSION

The relationship between GDNF and malignancy grade is unclear. Nevertheless, GDNF expression is higher in glioblastomas. Overall GDNF expression is increased in glioma cells with high migration activity. At the same time, αGDNF and βGDNF isoforms demonstrate higher expression in actively migrating cells that can indicate their participation in regulation of tumor migration properties. No αGDNF expression with simultaneous low βGDNF expression may be a prognostic sign of low migration activity of human glioma cells.

Keywords:

GDNF

isoforms

splice variants αGDNF and βGDNF

glioma

tumor cell migration

Authors:

Shamadykova D.V.

Institute of Higher Nervous Activity and Neurophysiology

ORCID: 0000-0003-3665-667X

Zakharova L.G.

Institute of Higher Nervous Activity and Neurophysiology

ORCID: 0009-0005-6812-5402

Pavlova S.A.

Institute of Higher Nervous Activity and Neurophysiology

ORCID: 0000-0002-9595-9358

Pavlova G.V.

Institute of Higher Nervous Activity and Neurophysiology;
Burdenko Neurosurgical Center

SPIN RSCI: 4633-8339
Scopus AuthorID: 7005650683
ORCID: 0000-0002-6885-6601

Received:

13.09.2024

Accepted:

20.09.2024

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