Connexin-43 antibodies in intraoperative diagnosis of experimental poorly differentiated gliomas

Iusubalieva G.M.; Zorkina Ia.A.; Baklaushev V.P.; Gurina O.I.; Goriaĭnov S.A.; Aleksandrova E.V.; Zhukov V.Iu.; Savel'eva T.A.; Potapov A.A.; Chekhonin V.P.

doi:https://doi.org/

Iusubalieva G.M.

Otdel fundamental'noĭ i prikladnoĭ neĭrobiologii FGBU "Gosudarstvennyĭ nauchnyĭ tsentr sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo";
Kafedra meditsinskikh nanobiotekhnologiĭ Rossiĭskogo gosudarstvennogo meditsinskogo universiteta im. N.I. Pirogova

Zorkina Ia.A.

Otdel fundamental'noĭ i prikladnoĭ neĭrobiologii FGBU "Gosudarstvennyĭ nauchnyĭ tsentr sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo"

Baklaushev V.P.

Gurina O.I.

Laboratoriia neĭrokhimii otdela fundamental'noĭ i prikladnoĭ neĭrobiologii Gosudarstvennogo nauchnogo tsentra sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo

Goriaĭnov S.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Aleksandrova E.V.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Zhukov V.Iu.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN

Savel'eva T.A.

Institut obshcheĭ fiziki im. A.M. Prokhorova RAN

Potapov A.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Chekhonin V.P.

Connexin-43 antibodies in intraoperative diagnosis of experimental poorly differentiated gliomas

Authors:

Iusubalieva G.M., Zorkina Ia.A., Baklaushev V.P., Gurina O.I., Goriaĭnov S.A., Aleksandrova E.V., Zhukov V.Iu., Savel'eva T.A., Potapov A.A., Chekhonin V.P.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2014;78(3): 3‑13

Read: 1122 times

To cite this article:

Iusubalieva GM, Zorkina IaA, Baklaushev VP, et al. . Connexin-43 antibodies in intraoperative diagnosis of experimental poorly differentiated gliomas. Burdenko's Journal of Neurosurgery. 2014;78(3):3‑13. (In Russ., In Engl.)

Подписка 2025 Журнал «Вопросы нейрохирургии» имени Н.Н. Бурденко (Burdenko's Journal of Neurosurgery)

Использование инфографики авторами научных работ

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Abstract:

Fluorescent diagnosis was first proposed in the early XX century and has been used in neurosurgery for about 15 years. The method relies on selective accumulation of strongly fluorescent protoporphyrin IX in tumor cells. Over the past years, the method of intraoperative fluorescence diagnosis has occupied its niche in many neurosurgical clinics around the world and is now used for fast intraoperative diagnosis in brain tumor surgery. However, the efficiency of fluorescent intraoperative diagnosis using 5-aminolevulinic acid is 80-90% and 58.8% for surgery of Grade III-IV and I-II gliomas, respectively. One of the methods to improve the efficiency of fluorescent diagnosis is to use vector systems for delivering fluorescent drugs into the tumor. This paper reports the results of an experimental study of systems for delivering fluorescent agents (protoporphyrin IX, Alexa 488, Alexa 660) using connexin-43 antibodies in rats with transplanted C6 glioma.

Keywords:

connexin-43

5- aminolevulinic acid

glioma

connexin-43

fluorescent diagnosis

5- aminolevulinic acid

laser spectroscopy

glioma

fluorescent diagnosis

laser spectroscopy

Authors:

Iusubalieva G.M.

Zorkina Ia.A.

Otdel fundamental'noĭ i prikladnoĭ neĭrobiologii FGBU "Gosudarstvennyĭ nauchnyĭ tsentr sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo"

Baklaushev V.P.

Gurina O.I.

Laboratoriia neĭrokhimii otdela fundamental'noĭ i prikladnoĭ neĭrobiologii Gosudarstvennogo nauchnogo tsentra sotsial'noĭ i sudebnoĭ psikhiatrii im. V.P. Serbskogo

Goriaĭnov S.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Aleksandrova E.V.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Zhukov V.Iu.

FGBU "NII neĭrokhirurgii im. akad. N.N. Burdenko" RAMN

Savel'eva T.A.

Institut obshcheĭ fiziki im. A.M. Prokhorova RAN

Potapov A.A.

NII neĭrokhirurgii im. akad. N.N. Burdenko RAMN, Moskva

Chekhonin V.P.

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References:

Goryainov S.A. Intraoperative fluorescent diagnosis and laser spectroscopy in surgery of brain gliomas. Summary of the Candidate of Medicine Thesis. Moscow 2013. In Russian.
Ermakova K.V. Photodynamic therapy of brain tumors in rats using domestic photosensitizers. Summary of the Candidate of Medicine Thesis. Moscow 2010. In Russian.
Kobyakov G.L. Chemotherapy in complex treatment of patients with primary malignant brain tumors. Summary of the Doctor of Medicine Thesis. Moscow 2011. In Russian.
Konovalov A.N., Potapov A.A., Gavrilov A.G. et al. Modern technologies in neurosurgery. In: Modern technologies and clinical research in neurosurgery. Ed. by A.N. Konovalov. Moscow: 2012; 1: 2: 55-113. In Russian.
Malkarov M.S., Dreval’ O.N., Borzunov A.N. et al. Methods of intraoperative control during removal of intracerebral brain tumors. Vopr Neirokhir 2010; 3: 20-25. In Russian.
Parfenov V.E., Svistov D.V., Savello A.V., Lapshin R.A., Tsibirov A.V. Intra-operative ultrasound navigation in surgical treatment of brain tumors. Ros Neirokhir 2004; 2: 13: 6-11. In Russian.
Potapov A.A., Gavrilov A.G., Goryainov S.V., Gol’bin D.A., Zelenkov P.V., Kobyakou G.L., Okhlopkov V.A., Zhukov V.Yu., Shishkina L.V., Shurkhai V.A., Loshchenov V.B., Savel’eva T.A., Grachev P.V., Holodtsova M.N., Kuz’min S.G., Vorozhtsov G.N. Intraoperative fluorescent diagnosis and laser spectroscopy in surgery of glial brain tumors. Vopr Neirokhir 2012; 5: 3-12. In Russian.
Potapov A.A., Gavrilov A.G., Zelenkov P.V., Okhlopkov V.A., Shishkina L.V., Goryainov S.A., Shurkhai V.A., Loshchenov V.B., Savel’eva T.A., Grachev P.V., Holodtsova M.N., Kuz’min S.G., Gol’bin D.A. Use of fluorescent diagnosis and laser spectral analysis in surgery of intracranial meningiomas. Vopr Neirokhir 2012; 4: 12-18. In Russian.
Baklaushev V.P., Gurina O.I., Yusubalieva G.M.,Grinenko N.F., Cytrin E.B., Victorov I.V., Chekhonin V.P. Immunofluorescent analysis of connexin-43 using monoclonal antibodies to its extracellular domain. Bull Exp Biol Med 2009; 148: 4: 725-730.
Baklaushev V.P., Yusubalieva G.M., Tsitrin E.B., Gurina O.I., Grinenko N.F., Victorov I.V., Chekhonin V.P. Visualization of connexin-43-positive cells of glioma and the periglioma zone by means of intravenously injected monoclonal antibodies. Drug Deliv 2011; 18: 5: 331-337.
Black P.M.L., Moriarty ?., Alexander E. et al. Development and implementation of intraoperative magnetic resonance imaging and its neurosurgical applications. Neurosurgery 1997; 41: 831-845.
Coluccia D., Fandino J., Fujioka M., Cordovi S., Muroi C., Landolt H. Intraoperative 5-aminolevulinic-acid-induced fluorescence in meningiomas. Acta Neurochir (Wien) 2010; 152: 10: 1711-1719.
Floeth F.W., Sabel M., Ewelt C., Stummer W., Felsberg J., Reigenberger G., Steiger H.J., Stoffels G., Coenen H.H., Langen K.J. Comparison of (18)F-FET PET and 5-ALA fluorescence in cerebral gliomas. Eur J Nucl Med Mol Imaging 2011; 38: 4: 731-741.
Iizuka Y., Kojima H., Kobata T., Kawase T., Kawakami Y., Tode M. Indentification of a glioma antigen, GARC-1, using cytotoxic T-lymphocytes induced by HSV cencer vaccine. Int J Cancer 2006; 118: 4: 942-949.
Kajimoto Y., Kuroiwa T., Miyatake S., Ichioka T., Miyashita M., Tanaka H., Tsuji M. Use of 5-aminolevulinic acid in fluorescence-guided resection of meningioma with high risk of recurrence. Case report. J Neurosurg 2007; 106: 6: 1070-1074.
Kamp M.A., Grosser P., Felsberg J., Slotty P.J., Steiger H.J., Reifenberger G., Sabel M. 5-aminolevulinic acid (5-ALA)-induced fluorescence in intrace-rebral metastases: a retrospective study. Acta Neurochir (Wien) 2012; 154: 2: 223-228; discussion 228.
Kostron H., Bauer R. Management of recurres strategies. Wien Med Wochenschr 2011; 161: 1-2: 20-21.
Leighton C., Fisher B., Bauman G., Depiero S., Stitt L., MacDonald D., Cairncross G. Supratentorial low-grade glioma in adults: an analysis of prognostic factor and timing of radiation. J Clin Oncol 1997; 15: 4: 1294-1301.
Miyatake S., Kajimoto Y., Kuroiwa T. Intraoperative photodynamic diagnosis of brain tumors. Brain Nerve 2009; 61: 7: 835-842. (Review Japanese Valdes et al., 2011).
Ohashi Y., Ueda M., Kawase T., Kawakami Y., Toda M. Identification of an epigenetically silenced gene, RFX1, in human glioma cells using restriction landmark genomic scanning. Oncogene 2004; 23: 47: 7772-7779. (Ueda R. et al., 2004).
Oliveira R., Christo Christov, Guillamo J.S., de Bouard S.P., Lalfi S., Vanance L., Tardy M., Peschanski M. Contribution of gap junctional communication between tumor cells and astroglia to the invasion of the brain parenchyma by human Glioblastomas BMC. Cell Biol 2005; 6: 7.
Potapov A.A., Loshakov V.A., Usachev D.J. et al. Intaoperative multimodal navigation including laser fluorescence spectroscopy in surgery of malignant brain tumors. Materials of 14th European Congress of Neuosurgery. Rome (Italy) 2011.
Potapov A.A., Loshakov V.A., Usachev D.U. et al. Multimodal navigation including laser spectroscopy in surgery of intracerebral tumors. Neurosurgery and Neurology of Kazakhstan 2011; 2: 23: 6.
Potapov A.A., Usachev D.J., Loshakov V.A. et al. First experience in 5-ALA-fluorescence-guided and endoscopically assisted microsurgery of brain tumors. Med Las Appl 2008; 23: 4: 202-208.
Sanai N., Berger M.S. Extent of resection influences outcomes for patients with gliomas. Rev Neurol (Paris) 2010; 167: 10: 648-654. doi: 10.1016/j.neurol.2011.07.004.
Stepp H., Beck T., Pongratz T., Meinel T., Kreth F.W., Tonn J.Ch., Stummer W. ALA and malignant glioma: fluorescence-guided resection and photodynamic treatment. J Environ Pathol Toxicol Oncol 2007; 26: 2: 157-164.
Stummer W., Novotny A., Stepp H., Goetz C., Bise K., Reulen H.J. Fluorescence guided resection of glioblastoma multiforme by using 5-aminolevulinic acid-induced porphyrins: a prospective study in 52 consecutive patients. N Neurosurg 2000; 93: 6: 1003-1013.
Stummer W., Stepp H., Moller G., Ehrhardt A., Leonhard M., Reulen H.J. Technical principles for protoporphyrin-IX-fluorescence guided microsurgical resection of malignant glioma tissue. Acta Neurochir (Wien) 1998; 140: 10: 995-1000.
Stummer W., Pilchmeier U., Meinel T. et al. Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomized controlled multicentre phase III trial. Lancet Oncol 2007; 7: 392-401.
Stupp R., Weber D.C. The role of radio- and chemotherapy in glioblastoma. Onkologie 2005; 28: 6-7: 315-317.
Ueda R., Yoshida K., Kawakami Y., Kawase T., Tode M. Immunohistoche-mical analysis of SOX6 expression in human brain tumors. Brain Tumor Pathol 2004; 21: 3: 117-120.
Ueda R., Yoshida K., Kawase T., Kawakami Y., Tode M. Preferential expression and frequent IgG responses of a tumor antigen, SOX5, in glioma patients. Int J Cancer 2007; 120: 8: 1704-1711.
Zhang W., Couldwell W.T., Simard M.F., Song H., Lin J.H., Nedergaard M. Direct gap junction communication between malignant gliomacells and astrocytes. Cancer Res 1999; 59: 8: 1994-2003.