The ratio of autophagy activity and protein resynthesis in patients undergoing a wellness program in combination with food deprivation

Lysenkov SP, Korchazhkina NB, Dautov YuYu, et al. The ratio of autophagy activity and protein resynthesis in patients undergoing a wellness program in combination with food deprivation. Problems of Balneology, Physiotherapy and Exercise Therapy. 2025;102(5‑2):6‑16. (In Russ.)
https://doi.org/10.17116/kurort20251020526

Подписка 2026 Вопросы курортологии, физиотерапии и лечебной физической культуры (Problems of Balneology, Physiotherapy and Exercise Therapy)

Использование инфографики авторами научных работ

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OBJECTIVE

To study the ratio of the activity of proteasome 20S and beclin-1 during CWP.

MATERIAL AND METHODS

The study is single-center, prospective, and continuous, and includes an examination of 48 patients aged 18 to 64 years who underwent caloric restriction (30—40% of the required amount) and therapeutic fasting. On the first and 12th days, the concentration of protein (pg/ml) of proteasome 20S and Beclin-1 in blood plasma was determined by the enzyme immunoassay (Cloud-CloneCorp test systems, USA), as well as body composition by the impedance method.

RESULTS

The optimal ratio of the base activity of 20S and autophagy was observed only in individuals with a BMI of 30 kg/m². CWP and low-calorie diet were accompanied by the establishment of a similar ratio in the entire group and separately in the group of women, as well as in patients with a BMI of 30 kg/m² and in people over 60 years of age. The source of proteasome activity was lean and lean muscle mass; for Beclin-1, the dominant components from the body composition were not detected. During therapeutic fasting, the optimal ratio of 20S and Beclin-1 activity was observed during periods 3—4 (the «silver fasting period») and 11—12 days. These time periods can be recommended for outpatient (3—4 days) and inpatient (11—12 days) medical practice.

CONCLUSION

CWP is accompanied by positive molecular effects aimed at activating autophagy and protein resynthesis. 20S activity and autophagy are affected by factors such as BMI, gender, age, and the degree of calorie restriction. CWP in combination with a low-calorie diet optimizes the ratio of protein resynthesis activity and autophagy in most patients, which contributes to the healing process.

Keywords:

wellness program

proteasome 20S

protein resynthesis

Beclin-1

autophagy

food deprivation

Authors:

Lysenkov S.P.

Maikop State Technological University

ORCID: 0000-0003-1179-8938

Korchazhkina N.B.

FSBSI «Petrovsky National Research Centre of Surgery»

SPIN RSCI: 9733-7646
ORCID: 0000-0002-9804-7725

Dautov Yu.Yu.

Maikop State Technological University

ORCID: 0009-0003-0732-9255

Tkhakushinov I.A.

Maikop State Technological University

ORCID: 0009-0005-0279-7417

Urakova T.Yu.

Maikop State Technological University

ORCID: 0000-0002-6572-745X

Muzhenya D.V.

Adyghe State University

ORCID: 0000-0002-4379-0634

Tkhakushinov R.A.

Maikop State Technological University

ORCID: 0000-0001-5868-6701

Chamokov E.V.

Maikop State Technological University

ORCID: 0000-0002-8600-738X

Pavlenko M.Yu.

Maikop State Technological University

ORCID: 0009-0006-5791-4016

Received:

22.10.2025

Accepted:

31.10.2025

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