Dynamics of activity of neurokinine receptors and enzymes of intercellular matrix (MMP9 and TIMP-2) in the nasal mucosa of the chronic cigarette smokers

Lepeĭko Iu.B.; Diuĭzen I.V.; Nevzorova V.A.; Gilifanov E.A.

doi:https://doi.org/

Lepeĭko Iu.B.

TsNIL Tikhookeanskogo gosudarstvennogo meditsinskogo universiteta, Vladivostok, Rossiia, 690105

Diuĭzen I.V.

TsNIL Tikhookeanskogo gosudarstvennogo meditsinskogo universiteta, Vladivostok, Rossiia, 690105

Nevzorova V.A.

Pacific State Medical University, Vladivostok, Russia

Gilifanov E.A.

Kafedra otorinolaringologii Vladivostokskogo gosudarstvennogo meditsinskogo universiteta Minzdrava Rossii

Dynamics of activity of neurokinine receptors and enzymes of intercellular matrix (MMP9 and TIMP-2) in the nasal mucosa of the chronic cigarette smokers

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Lepeĭko Iu.B., Diuĭzen I.V., Nevzorova V.A., Gilifanov E.A.

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Journal: Russian Bulletin of Otorhinolaryngology. 2014;(3): 12‑16

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To cite this article:

Lepeĭko IuB, Diuĭzen IV, Nevzorova VA, Gilifanov EA. Dynamics of activity of neurokinine receptors and enzymes of intercellular matrix (MMP9 and TIMP-2) in the nasal mucosa of the chronic cigarette smokers. Russian Bulletin of Otorhinolaryngology. 2014;(3):12‑16. (In Russ.)

Подписка 2026 Вестник оториноларингологии (Russian Bulletin of Otorhinolaryngology)

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Abstract:

The present study was designed to determine localization of substance P and neuroknine receptors in the nasal mucous membrane of the subjects having the history of chronic smoking. The mechanisms underlying reorganization of the mucous tissue were elucidated by measuring the activity of intercellular matrix enzymes (MMP9 and TIMP-2). The activity of neurokinine receptors localized on the cells of ciliary epithelium and in the connective tissue elements of the submucous structures was shown to be significantly increased. It is concluded that the specific dynamics of MMP9 and TIMP-2 activities in the chronic cigarette smokers indicates that the matrix metalloproteinases of this type play an important role in the structural reorganization of the tissues of the nasal mucous membrane.

Keywords:

neurogenic inflammation

neurokinine receptors

substance P

matrix metalloproteinases

tissue inhibitors of matrix metalloproteinases

nasal mucous membrane

Authors:

Lepeĭko Iu.B.

TsNIL Tikhookeanskogo gosudarstvennogo meditsinskogo universiteta, Vladivostok, Rossiia, 690105

Diuĭzen I.V.

TsNIL Tikhookeanskogo gosudarstvennogo meditsinskogo universiteta, Vladivostok, Rossiia, 690105

Nevzorova V.A.

Pacific State Medical University, Vladivostok, Russia

Gilifanov E.A.

Kafedra otorinolaringologii Vladivostokskogo gosudarstvennogo meditsinskogo universiteta Minzdrava Rossii

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References:

Vergnolle N., Alexandre Denadai-Souza, Laurence Martin, Marco A. Vieira de Paula, Maria C. Werneck de Avellar, Marcelo N. Muscará, Nicolas Cenac. A role for transient receptor potential vanilloid 4 in tonicity-induced neurogenic inflammation. Br J Pharmacol 2010; 5: 1161-1173.
Baraniuk J.N., Merck S.J. Neuroregulation of human nasal mucosa. Ann NY Acad Sci 2009; 604-609.
Nevzorova V.A., Gilifanov E.A. Rol' substantsii P v fiziologii I patologii organov dykhaniya I slukha [The role of substance P in physiology and pathology of the organs of respiration and hearing]. Ros otorinolar 2010; 5: 49-54.
Kwong K., Wu Z.X., Kashon M.L., Krajnak K.M., Wise P.M., Lee L.Y. Chronic smoking enhances tachykinin synthesis and airway responsiveness in guinea pigs. Am J Respir Cell Mol Biol 2001; 3: 299-305.
Baraniuk J.N. Neurogenic mechanisms in rhinosinusitis. Curr Allergy Asthma Rep 2001; 3: 252-261.
Jancso N., Jancso-Gabor A., Szolcsanyi J. Direct evidence for neurogenic inflammation and its prevention by denervation and by pretreatment with capsaicin. Br J Pharmacol 1967; 31: 138-151.
Andrè E., Campil B., Materazzi S., Trevisani1 M., Amadesi S., Massi D., Creminon C., Vaksman N., Nassini R., Civelli M., Pier Giovanni Baraldi, Daniel P. Poole, Nigel W., Bunnett, Pierangelo Geppetti, Patacchini R. Cigarette smoke-induced neurogenic inflammation is mediated by alpha, beta-unsaturated aldehydes and the TRPA1 receptor in rodents. J ClinInvest 2008; 7: 2574-2582.
O'Connor T., O'Connell J., O'Brien, Darren I., Triona Goode, Charles P. Bredin, Fergus Shanahan. The role of substance P in inflammatory disease. J Cell Physiol 2004; 201: 167-180.
Schmeltz M., Petersen L. Neurogenic inflammation in human and rodent skin. News Physiol Sci 2001; 16: 33-37.
Solov'eva N.I., Ryzhakova O.S. Metody opredeleniya aktivnosti matriksnykh metalloproteinaz [The methods for the evaluation of the activity of matrix metalloproteinases]. Klin lab diagn 2010; 2: 17-21.
Can I.H. et al. The expression of MMP-2, MMP-7, MMP-9, and TIMP-1 in chronic rhinosinusitis and nasal polyposis. Otolaryngol Head Neck Surg 2008; 2: 211-215.
Ziora D., Dworniczak S., Kozielski J. Induced sputum metalloproteinases and their inhibitors in relation to exhaled nitrogen oxide and sputum nitric oxides and other inflammatory cytokines in patients with chronic obstructive pulmonary disease. J Physiol Pharmacol 2008; 6: 809-817.
Soboleva G.M., Sukhikh G.T. Semeistvo matriksnykh metalloproteinaz: obshchaya kharacteristika I fiziologicheskaya rol' [The family of matrix metalloproteinases: the general characteristic and physiological role]. Akush I gin 2007; 1: 5-7.
Shoikhet Ya.N., Korenovsky Yu.V., Lepilov A.V., Motin Yu.G., Lel' N.V. Rol' matriksnykh metalloproteinaz pri vospalitel'nykh zabolevaniyakh legkikh [The role of matrix metalloproteinases in pulmonary inflammatory diseases]. Probl kiln med 2008; 3: 99-101.
López Y.S., Boado Yolanda, López-Boado S., Jun U. Li, Chris L. Clayton, Joanne L. Wright, Andrew Churg. Modification of the rat airway explanttranscriptome by cigarette smoke. Inhal Toxicol 2010; 3: 234-244.
Tsuchida H., Takahashi S., Nosaka E., Kuraya T., Yamashita M., Morimoto K. Novel triple neurokinin receptor antagonist CS-003 inhibits respiratory disease models in guinea pigs. Eur J Pharmacol 2008; 1-3: 153-159.
Samuel Santos Valença, Luís Cristóvão Porto. Immunohistochemical study of lung remodeling in mice exposed to cigarette smoke. J Bras Pneumol 2008; 34: 10: 787-795.
Santana A., Carlos Medina, Maria Cristina Paz-Cabrera, Federico Díaz-Gonzalez, Esther Farré, Antonio Salas, Marek W. Radomski. Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice. World J Gastroenterol 2006; 12: 6464-6472.
Nevzorova V.A., Gilifanov E.A., Tilik T.V. Kurenie tabaka I funktsional'no-morfologicheskoe modelirovanie slizistoy obolochki verkhnikh dykhatrl'nykh putey [Tobacco smoking and functional-morphological remodelling of the mucous membrane of the upper respiratory tract]. Ros otorinolr 2010; 4: 47: 115-120.
Tai C.F., Baraniuk J.N. Upper air way neurogenic mechanisms. Curr Opin Allergy Clin Immunol 2002; 1: 11-19.
Groneberg D.A., Nowak D., Wussow A., Fischer A. Toxic rhinitis-induced changes of human nasal mucosa innervation. Toxicol Pathol 2003; 3: 326-231.
Pfaar O., Raap U., Holz M., Hormann K., Klimek L. Pathophysiology of itching and sneezing in allergic rhinitis. Swiss Med Wkly 2009; 139: 35-40.
Geppetti P., Fusco BM., Marabini S., Maggi CA., Fanciullacci M., Sicuteri F. Secretion, pain and sneezing induced by the application of capsaicin to the nasal mucosa in man. Br J Pharmacol 1988; 3: 509-514.
Pawankar R., Nonaka М. Inflammatory mechanisms and remodeling in chronic rhinosinusitis and nasal polyps. Curr Allergy Asthma 2007; 3: 202-208.
Atkinson Jeffrey J., Senior Robert M. Matrix Metalloproteinase-9 in Lung Remodeling. Am J Respir Cell Mol Biol 2003; 12-24.
Nevzorova V.A., Panchenko E.A., Vakhrusheva S.E., Tilik T.V., Gilifanov E.A. Rol' matriksnykh metalloproteinaz v formirovanii morfofunktsional'nogo balansa vozdukhonosnykh putey pri khronicheskoy obstruktivnoy bolezni lyogkikh [The role of matrix metalloproteinases in the formation of morphofunctional balance in the airways of the patients presenting with chronic obstructive pulmonary disease]. Tikhookean med zhurn med 2011; 2: 9-13.
Lepeiko Yu.B., Gilifanov E.A., Dyuizen I.V. Dinamika aktivnosty MMP-9 i TIMP-2 v slizistoy obolochke nosa u krys pri eksperimental'nom modelirovanii khronicheskogo tabakokureniya [Dynamics of the activity of MMP-9 and TIMP-2 in the nasal mucous membrane of the rats during experimental modelling of chronic tobacco smoking]. Materiályixmezinárodní vědecko - praktická conference "DnyVědy 2013" 2013; 33-41.