Role of negative regulators of SOCS1, SOCS3, and SOCS5 gene transcription in the negative cell signaling regulation system in asthma

Sorokina L.N.; Mineev V.N.; Lim V.V.

doi:https://doi.org/10.17116/terarkh201789343-47

Sorokina L.N.

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, Saint Petersburg, Russia

Mineev V.N.

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, Saint Petersburg, Russia

Lim V.V.

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, Saint Petersburg, Russia

Role of negative regulators of SOCS1, SOCS3, and SOCS5 gene transcription in the negative cell signaling regulation system in asthma

Authors:

Sorokina L.N., Mineev V.N., Lim V.V.

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Journal: Therapeutic Archive. 2017;89(3): 43‑47

DOI: 10.17116/terarkh201789343-47

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To cite this article:

Sorokina LN, Mineev VN, Lim VV. Role of negative regulators of SOCS1, SOCS3, and SOCS5 gene transcription in the negative cell signaling regulation system in asthma. Therapeutic Archive. 2017;89(3):43‑47. (In Russ.)
https://doi.org/10.17116/terarkh201789343-47

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Abstract:

Aim. To conduct a comprehensive study of the components of negative cell signaling regulation in different types of asthma. Subjects and methods. A total of 171 people, including 80 patients with allergic asthma (AA), 60 patients with non-allergic asthma (NAA), and 31 apparently healthy individuals, were examined. SOCS5 mRNA expression was assessed by reverse-transcription polymerase chain reaction. The expression of SOCS1 and SOCS3 proteins was investigated by immunoblotting. The concentration of total serum IgE was determined by enzyme immunoassay; the level of cytokines was measured according to the standard protocol using a Bio-Plex fluorometer. Results. The findings show that the patients with AA generally display more marked changes in the expression of all three investigated SOCSes (SOCS1, SOCS3, and SOCS5) at baseline and when interleukin 4 (IL-4) acts. In NAA, there are pronounced changes in the expression of SOCS3 only and, to a lesser extent, SOCS5. The results of investigating the concentrations of IL-4 in the examined groups demonstrate its significant decrease in the AA group, whereas in the NAA group, it is similar to those in healthy individuals. On the contrary, IL-10 concentrations in AA tend towards those in the control group, but much exceed in NAA. Conclusion. The findings allow one to consider the complexity of regulatory disorders arising at various levels of cell signaling in the context of the multifunctional nature of the molecules from the family of negative regulators of transcription of the SOCS1, SOCS3, and SOCS5 genes, which provide the comprehensive control of cytokine signaling simultaneously in different signal pathways.

Keywords:

asthma

SOCS

SOCS1

SOCS3

SOCS5 protein system

mononuclear cells

Authors:

Sorokina L.N.

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, Saint Petersburg, Russia

Mineev V.N.

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, Saint Petersburg, Russia

Lim V.V.

I.P. Pavlov First Saint Petersburg State Medical University, Ministry of Health of Russia, Saint Petersburg, Russia

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References:

Mineev VN, Sorokina LN, Trofimov VI. Fundamental`nye i klinicheskie aspekty JAK-STATsignalizatsii [The fundamental and clinical aspects of the JAK-STAT signaling]. St. Petersburg: BBM; 2010. (In Russ.)
Fujimoto M, Naka T. Regulation of cytokine signaling by SOCS family molecules. Trends in Immunology. 2003;24(12):659-665. doi:10.1016/j.it.2003.10.008
Fukuyama S, Nakano T, Matsumoto T, Oliver BG, Burgess JK, Moriwaki A, Tanaka K, Kubo M, Hoshino T, Tanaka H, McKenzie AN, Matsumoto K, Aizawa H, Nakanishi Y, Yoshimura A, Black JL, Inoue H. Pulmonary suppressor of cytokine signaling-1 induced by IL-13 regulates allergic asthma phenotype. American Journal of Respiratory and Critical Care Medicine. 2009;179(11):992-998. doi:10.1164/rccm.200806-992OC
Albanesi C, Fairchild H, Madonna S, Scarponi C, De Pità O, Leung DY, Howell MD. IL-4 and IL-13 negatively regulate TNF-α- and IFN-γ-induced β-defensin expression through STAT6, suppressor of cytokine signaling SOCS1 and SOCS3. The Journal of Immunology. 2007;179(2):984-992. doi:10.4049/jimmunol.179.2.984
Kubo M, Inoue H. Suppressor of cytokine signaling 3 (SOCS3) in Th2 cells evokes Th2 cytokines, IgE, and eosinophilia. Current Allergy and Asthma Reports. 2006;6(1):32-39. doi:10.1007/s11882-006-0007-6
Seki Y, Inoue H, Nagata N, Hayashi K, Fukuyama S, Matsumoto K, Komine O, Hamano S, Himeno K, Inagaki-Ohara K, Cacalano N, O'Garra A, Oshida T, Saito H, Johnston JA, Yoshimura A, Kubo M. SOCS-3 regulates onset and maintenance of TH2-mediated allergic responses. Nature Medicine. 2003;9(8):1047-1054. doi:10.1038/nm896
Lopez E, Paz Zafra M, Sastre B, Gámez С, Fernández-Nieto M, Sastre J, Lahoz C, Quirce S, Del Pozo V. Suppressors of cytokine signaling 3 expression in eosinophils: Regulation by PGE2 and Th2 Cytokines. Clinical and Developmental Immunology. 2011;2011:3-11. doi:10.1155/2011/917015
Christodoulopoulos P, Cameron L, Nakamura Y, Lemière C, Muro S, Dugas M, Boulet L-P, Laviolette M, Olivenstein R, Hamid Q. Th2 cytokine-associated transcription factors in atopic and nonatopic asthma: evidence for differential signal transducer and activator of transcription 6 expression. Journal of Allergy and Clinical Immunology. 2001;107(4):586-591. doi:10.1067/mai.2001.114883
Daewoong J, Liu D, Yao S, Collins RD, Hawiger J. Intracellular protein therapy with SOCS3 inhibits inflammation and apoptosis. Nature Medicine. 2005;11(8):892-898. doi:10.1038/nm1269