Characteristics of stromal cell precursors in patients after allogeneic bone marrow transplantation

Petinati N.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Shipunova I.N.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Bigil'deev A.E.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Kuz'mina L.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava Rossii, Moskva

Saribekian R.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Gal'tseva I.V.

GNTs Minzdrava Rossii, Moskva

Misiurin A.V.

Federal'nyĭ nauchno-klinicheskiĭ tsentr detskoĭ gematologii, onkologii i immunologii im. Dmitriia Rogacheva Minzdrava Rossii, Moskva

Parovichnikova E.N.

Federal state budgetary institution 'Hematological Research Centre', Russian Ministry of Health, Moscow, Russia

Savchenko V.G.

National Research Center for Hematology, Moscow, Russia

Drize N.I.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Characteristics of stromal cell precursors in patients after allogeneic bone marrow transplantation

Authors:

Petinati N.A., Shipunova I.N., Bigil'deev A.E., Kuz'mina L.A., Saribekian R.A., Gal'tseva I.V., Misiurin A.V., Parovichnikova E.N., Savchenko V.G., Drize N.I.

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Journal: Therapeutic Archive. 2013;85(12): 95‑99

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To cite this article:

Petinati NA, Shipunova IN, Bigil'deev AE, et al. . Characteristics of stromal cell precursors in patients after allogeneic bone marrow transplantation. Therapeutic Archive. 2013;85(12):95‑99. (In Russ.)

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Aim. To study the elements of the mesenchymal stromal cell compartment (multipotent mesenchymal stromal cells (MMSCs)) and their more mature progenies of fibroblast colony-forming units (CFU-F) in patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Subjects and methods. The total production of MMSCs after 5 passages, the time of their growth, and the concentration of CFU-F in the bone marrow from patients were determined using the control sections before transplantation and over time for 2 years after allo-HSCT. What is more, the genetic affiliation of the MMSCs from the patients after allo-HSCT and their immunophenotype were studied. Results. The MMSCs from the patients after allo-HSCT belong to a recipient and have the immunophenotype that meets the international standard for these cells. The total production of MMSCs in the cultures obtained from the bone marrow of the patients with hematologic diseases was decreased. Not all the samples from the patients after allo-HSCT are able to undergo 5 passages. In addition, the time of growth substantially increases and the total production of cells decreases in all the analyzed cultures. These indicators are gradually restored; however, they never achieve the mean values in donors. The concentration of CFU-F in the bone marrow from the patients are reduced as compared to that in the donors prior to transplantation and decreased still further after allo-HSCT. These cell precursors are not restored for at least 2 years following allo-HSCT. Conclusion. Both examined categories of the cell precursors of the stromal environment suffer from both the disease itself and allo-HSCT in the patients with hematologic diseases.

Keywords:

allogeneic hematopoietic stem cell transplantation

multipotent mesenchymal stromal cells

hemoblastoses

fibroblast colony-forming units

allogeneic hematopoietic stem cell transplantation

multipotent mesenchymal stromal cells

hemoblastoses

fibroblast colony-forming units

Authors:

Petinati N.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Shipunova I.N.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Bigil'deev A.E.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Kuz'mina L.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava Rossii, Moskva

Saribekian R.A.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

Gal'tseva I.V.

GNTs Minzdrava Rossii, Moskva

Misiurin A.V.

Federal'nyĭ nauchno-klinicheskiĭ tsentr detskoĭ gematologii, onkologii i immunologii im. Dmitriia Rogacheva Minzdrava Rossii, Moskva

Parovichnikova E.N.

Federal state budgetary institution 'Hematological Research Centre', Russian Ministry of Health, Moscow, Russia

Savchenko V.G.

National Research Center for Hematology, Moscow, Russia

Drize N.I.

Gematologicheskiĭ nauchnyĭ tsentr Minzdrava RF, Moskva

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