Determination of abacavir in biological objects for the purpose of chemical and toxicological analysis

Illarionova E.A.

Irkutsk State Medical University

ORCID: 0000-0002-3281-9489

Chmelevskaya N.V.

Irkutsk Regional Bureau of Forensic Medical Examination

ORCID: 0000-0002-1130-6262

Gonchikova Yu.A.

Irkutsk State Medical University

ORCID: 0000-0002-2964-3830

Mitina A.E.

Irkutsk State Medical University

Determination of abacavir in biological objects for the purpose of chemical and toxicological analysis

Authors:

Illarionova E.A., Chmelevskaya N.V., Gonchikova Yu.A., Mitina A.E.

More about the authors

Journal: Forensic Medical Expertise. 2022;65(2): 29‑33

DOI: 10.17116/sudmed20226502129

Downloaded: 12

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To cite this article:

Illarionova EA, Chmelevskaya NV, Gonchikova YuA, Mitina AE. Determination of abacavir in biological objects for the purpose of chemical and toxicological analysis. Forensic Medical Expertise. 2022;65(2):29‑33. (In Russ.)
https://doi.org/10.17116/sudmed20226502129

Подписка 2025-2 (Forensic Medical Expertise)

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Purpose — to develop a technique for isolating abacavir, as well as its detection and quantification in biological objects. Extraction of abacavir from centrifuges was carried out with chloroform at pH 8 in the presence of an electrolyte saturated solution of (NH₄)₂SO₄ once for 3 minutes. The determination of abacavir in extracts from urine, saliva and liver was carried out by thin-layer chromatography in the system of ethyl acetate: trichloromethane: ammonia, a concentrated solution of 25% (17:4:1) ascending method, UV spectrophotometry in a medium of hydrochloric acid 0.1 M, where the absorption spectrum abacavir is characterized by an absorption maximum at a wavelength of 297±1 nm, high performance liquid chromatography, during which one peak with a retention time of 9.5 min was observed on the chromatogram of a standard sample, which coincided with the retention time of abacavir obtained after extraction from biological objects.

Keywords:

abacavir

liquid-liquid extraction

solid-phase extraction

spectrophotometry

high performance liquid chromatography

Authors:

Illarionova E.A.

Irkutsk State Medical University

ORCID: 0000-0002-3281-9489

Chmelevskaya N.V.

Irkutsk Regional Bureau of Forensic Medical Examination

ORCID: 0000-0002-1130-6262

Gonchikova Yu.A.

Irkutsk State Medical University

ORCID: 0000-0002-2964-3830

Mitina A.E.

Irkutsk State Medical University

Received:

05.07.2021

Accepted:

27.07.2021

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