Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair

Starovoitova M.K.; Minachenkova A.S.; Krys’ko M.V.; Slustovskaya Yu.V.; Strelova O.Yu.; Kuklin V.N.

doi:https://doi.org/10.17116/sudmed20206303123

Starovoitova M.K.

St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia

Minachenkova A.S.

St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia

Krys’ko M.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Slustovskaya Yu.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Strelova O.Yu.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Kuklin V.N.

Sankt-Peterburgskaia gosudarstvennaia khimiko-farmatsevticheskaia akademiia Minzdrava RF

Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair

Authors:

Starovoitova M.K., Minachenkova A.S., Krys’ko M.V., Slustovskaya Yu.V., Strelova O.Yu., Kuklin V.N.

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Journal: Forensic Medical Expertise. 2020;63(3): 23‑29

DOI: 10.17116/sudmed20206303123

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Starovoitova MK, Minachenkova AS, Krys’ko MV, Slustovskaya YuV, Strelova OYu, Kuklin VN. Comparative characteristics of enzymatic hydrolysis methods for isolating toxic substances from blood and hair. Forensic Medical Expertise. 2020;63(3):23‑29. (In Russ.)
https://doi.org/10.17116/sudmed20206303123

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The aim of study was to develop methods for the hydrolysis with hyaluronidases for the isolation of drugs (phenobarbital, diphenhydramine hydrochloride and phenibut) from biological objects (blood, hair) and to compare their effectivenes with previously developed methods of enzymatic hydrolysis. The studies were carried out using model «blood — model drug substance (MDS)», a natural and artificially colored wool (hair) of laboratory animals — guinea pigs of white, red and black natural colors, which were daily given a solution of MDS and then were subjected to cosmetic effects — coloring. The isolation of MDS from the model complex «blood — MDS» and from wool was carried out using the developed methods of hydrolysis with proteolytic enzymes (papain, chymopsin and chymotrypsin) and hyaluronidase. Phenobarbital and diphenhydramine from hydrolysates were extracted by liquid-liquid extraction method, phenibut — by direct freezing extraction with acetonitrile. The analysis of the extracts was carried out by gas chromatography with mass selective detection. The results showed that the developed methods of enzymatic hydrolysis can be recommended for isolating substances of various properties (acidic, amphoteric and alkaline) from blood, natural and artificially colored wool (hair).

Keywords:

enzymatic hydrolysis

chymopsin

chymotrypsin

papain

hyaluronidases

blood

natural and artificially colored hair

phenobarbital

diphenhydramine

phenibut

Authors:

Starovoitova M.K.

St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia

Minachenkova A.S.

St. Petersburg State Chemical and Pharmaceutical University, St. Petersburg, Russia

Krys’ko M.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Slustovskaya Yu.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Strelova O.Yu.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Kuklin V.N.

Sankt-Peterburgskaia gosudarstvennaia khimiko-farmatsevticheskaia akademiia Minzdrava RF

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