Determination of the fluorescence intensity of coenzymes NADH and FAD in the skeletal muscle of the rat depending on the post-mortem interval

Babkina A.S.; Sundukov D.V.; Golubev A.M.; Ryzhkov I.A.; Tsokolaeva Z.I.; Zarzhetsky Yu.V.

doi:https://doi.org/10.17116/sudmed20206301131

Babkina A.S.

Department of Forensic Medicine, Medical Institute of the Peoples’ Friendship University of Russia, Moscow, Russia, 117198;
General resuscitation Research Center of the V.A. Negovsky Federal Scientific and Clinical Center of resuscitation and rehabilitation, Moscow, Russia, 107031

Sundukov D.V.

ORCID: 0000-0001-8173-8944

Golubev A.M.

Ryzhkov I.A.

Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia, 107301

Tsokolaeva Z.I.

General resuscitation Research Center of the V.A. Negovsky Federal Scientific and Clinical Center of resuscitation and rehabilitation, Moscow, Russia, 107031

Zarzhetsky Yu.V.

Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia, 107301

Determination of the fluorescence intensity of coenzymes NADH and FAD in the skeletal muscle of the rat depending on the post-mortem interval

Authors:

Babkina A.S., Sundukov D.V., Golubev A.M., Ryzhkov I.A., Tsokolaeva Z.I., Zarzhetsky Yu.V.

More about the authors

Journal: Forensic Medical Expertise. 2020;63(1): 31‑35

DOI: 10.17116/sudmed20206301131

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To cite this article:

Babkina AS, Sundukov DV, Golubev AM, Ryzhkov IA, Tsokolaeva ZI, Zarzhetsky YuV. Determination of the fluorescence intensity of coenzymes NADH and FAD in the skeletal muscle of the rat depending on the post-mortem interval. Forensic Medical Expertise. 2020;63(1):31‑35. (In Russ.)
https://doi.org/10.17116/sudmed20206301131

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

Aim of the study is to identify patterns of variations of the fluorescence intensity of NADH (reduced nicotinamide adenine dinucleotide) and FAD (oxidized flavin adenine dinucleotide) in the skeletal muscle depending on the time since death. For the evaluation of fluorescence intensity of the studied coenzymes, laser-induced spectroscopy in situ was used. We revealed the dynamic of the fluorescence intensity of NADH and FAD in the skeletal muscle of a rat at different times during the post-mortem period, and theoretically justified the observed phenomena. The results obtained allow us to consider the studied indicators as a potential criterion for determining the post-mortem interval

Keywords:

post-mortem interval

fluorescence

coenzymes

NADH

FAD

Authors:

Babkina A.S.

Sundukov D.V.

ORCID: 0000-0001-8173-8944

Golubev A.M.

Ryzhkov I.A.

Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia, 107301

Tsokolaeva Z.I.

General resuscitation Research Center of the V.A. Negovsky Federal Scientific and Clinical Center of resuscitation and rehabilitation, Moscow, Russia, 107031

Zarzhetsky Yu.V.

Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia, 107301

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