The detection of amphetamines in urine samples with the use of immunochromatographic test strips

Shanin I.A.; Khan O.Iu.; Petukhov A.E.; Smirnov A.V.; Eremin S.A.

doi:https://doi.org/

Shanin I.A.

Kafedra khimicheskoĭ énzimologii khimicheskogo fakul'teta MGU im. M.V. Lomonosova

Khan O.Iu.

Kafedra khimicheskoĭ énzimologii khimicheskogo fakul'teta MGU im. M.V. Lomonosova

Petukhov A.E.

I.M. Sechenov First Moscow Medical University, 8/2 Malaya Trubetskaya St., Moscow, Russian Federation, 119991;
Moscow Research and Practical Center for Narcology, Department of Public Health, 37/1 Lyublinskaya St., Moscow, Russian Federation, 109390

Smirnov A.V.

Volgograd State Medical University, Volgograd, Russia

ORCID: 0000-0001-5351-6105

Eremin S.A.

"Detskaia gorodskaia poliklinika #17", Sankt-Peterburg

The detection of amphetamines in urine samples with the use of immunochromatographic test strips

Authors:

Shanin I.A., Khan O.Iu., Petukhov A.E., Smirnov A.V., Eremin S.A.

More about the authors

Journal: Forensic Medical Expertise. 2012;55(4): 33‑37

Read: 9509 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Shanin IA, Khan OIu, Petukhov AE, Smirnov AV, Eremin SA. The detection of amphetamines in urine samples with the use of immunochromatographic test strips. Forensic Medical Expertise. 2012;55(4):33‑37. (In Russ.)

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Development of a method for laboratory diagnostics of acute oral FLY agaric poisoning by HPLC-MS/MS in urine analysis. Forensic Medical Expertise. 2026;(1):22-25

Abstract:

This study has demonstrated the possibility of using immunochromatographic test strips for the reliable qualitative detection of amphetamine and methamphetamine in the urine samples at a cut-off level of 300 ng/ml. The test strips obtained from different manufactures are shown to be slightly different in terms of specificity as appears from the frequency of cross-reactions with various pharmaceutical products and narcotic drugs. Also, the use of the immunochromatographic strips makes it possible to determine amphetamine in a range of concentrations from 100 to 1000 ng/ml by measuring the intensity of test-line colour with the help of a TotalLab TL120 programmer and special scanning programs. The analysis for amphetamine using the NrcoStop (Osiris S) immunochromatographic strips failed to confirm the presence of this substance in the urine samples from the subjects who had drunk 0.5 l of energy drinks, such as Adrenaline RUSH, Red Bull, and Burn. It means that the presence of amphetamine in the urine should not be attributed to the consumption of such drinks.

Keywords:

amphetamine

methamphetamine

immunochromatography

test strips

urine

Authors:

Shanin I.A.

Kafedra khimicheskoĭ énzimologii khimicheskogo fakul'teta MGU im. M.V. Lomonosova

Khan O.Iu.

Kafedra khimicheskoĭ énzimologii khimicheskogo fakul'teta MGU im. M.V. Lomonosova

Petukhov A.E.

Smirnov A.V.

Volgograd State Medical University, Volgograd, Russia

ORCID: 0000-0001-5351-6105

Eremin S.A.

"Detskaia gorodskaia poliklinika #17", Sankt-Peterburg

Close metadata

References:

Kollinz U.P. Novye metody immunoanaliza: Per. s angl. Pod red. A.M. Egorova. M: Mir 1991; 280.
Colbert D.L., Gallacher G., Mainwaring-Burton R.W. Single-reagent polarization fluoroimmunoassay for amphetamine in urine. Clin Chem 1985; 31: 7: 1193-1195.
Eremin S.A., Zaitchev S.V., Egorov A.M., Ermakov A.N., Smirnov A.V., Izotov B.N. Polarization fluoroimmunoassays for the specific determination of amphetamine and methamphetamine in urine with the Abbott TDx-analyzer. Anal Chim Acta 1989; 227: 287-290.
Demerchyan Sh.A. Tverdofaznyi immunofermentnyi metod opredeleniya amfetaminov v moche. Klin i lab diagnost 2007; 12: 20-22.
Bryun E.A., Myagkova M.A. Metod rannego vyyavleniya potrebleniya narkoticheskikh veshchestv "Dianark" dlya preduprezhdeniya narkomanii. M 2010; 30.
Dzantiev B.B., Zherdev A.V., Popov V.O., Vengerov Yu.Yu., Starovoitova T.A., Toguzov R.T. Sistemy ekspressnoi immunodetektsii biologicheski aktivnykh soedinenii. Klin i lab diagnost 2002; 8: 25-32.
Toksikologicheskaya khimiya. Metabolizm i analiz narkoticheskikh toksikantov: uchebnoe posobie. Pod red. N.I. Kaletinoi. M: GEOTAR-Media 2008; 308-314.