Development of a method for laboratory diagnostics of acute oral FLY agaric poisoning by HPLC-MS/MS in urine analysis

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Evdokimova E.A.

St. Petersburg State Chemical and Pharmaceutical University;
Scientific and Clinical Center of Toxicology named after Academician S.N. Golikov of the Federal Medical and Biological Agency

ORCID: 0009-0001-6759-526X

Balabanova O.L.

St. Petersburg State Chemical and Pharmaceutical University;
St. Petersburg Research Institute of Emergency Medicine named after. I.I. Dzhanelidze

ORCID: 0000-0002-8636-9858

Kuznetsova E.A.

Scientific and Clinical Center of Toxicology named after Academician S.N. Golikov of the Federal Medical and Biological Agency

ORCID: 0009-0008-7670-553X

Strelova O.Yu.

St. Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0001-6737-1023

Tursunov A.N.

Saint-Petersburg State University

ORCID: 0009-0007-3670-7576

Development of a method for laboratory diagnostics of acute oral FLY agaric poisoning by HPLC-MS/MS in urine analysis

Authors:

Evdokimova E.A., Balabanova O.L., Kuznetsova E.A., Strelova O.Yu., Tursunov A.N.

More about the authors

Journal: Forensic Medical Expertise. 2026;68(1): 22‑25

DOI: 10.17116/sudmed20266901122

Read: 361 times

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To cite this article:

Evdokimova EA, Balabanova OL, Kuznetsova EA, Strelova OYu, Tursunov AN. Development of a method for laboratory diagnostics of acute oral FLY agaric poisoning by HPLC-MS/MS in urine analysis. Forensic Medical Expertise. 2026;68(1):22‑25. (In Russ.)
https://doi.org/10.17116/sudmed20266901122

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

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Abstract:

OBJECTIVE

To develop a method for laboratory diagnostics of acute poisoning with fly agarics using liquid tandem chromatograph mass spectrometry in urine analysis.

MATERIAL AND METHODS

Identification was performed on a Nexera XR liquid chromatograph with an LCMS-8050 tandem mass spectrometer (Shimadzu, Japan) using a Shim-pack FC-ODS column, derivatization was performed by bimolecular dansylation. The object of the study was urine of patients of the Acute Poisoning Center of the State Budgetary Institution Research Institute of Emergency Medicine named after I.I. Dzhanelidze with a diagnosis of «Acute oral poisoning with fly agarics».

RESULTS

It was established that urine is a suitable object for chemical-toxicological analysis in case of poisoning with fly agarics by the method of bimolecular dansylation, ion-chromatograms of ibotenic acid and muscimol, as well as a spectrum of ion-products of toxicants were obtained. The peak retention time (RT) for muscimol was 7.5 min, for ibotenic acid 6.25 min.

CONCLUSION

As a result of the study, a method for the identification of ibotenic acid and muscimol in urine using dansyl chloride derivatization by HPLC-MS/MS was developed.

Keywords:

urine

HPLC-MS/MS

muscimol

ibotenic acid

fly agaric

panther fly agaric

poisoning

Authors:

Evdokimova E.A.

St. Petersburg State Chemical and Pharmaceutical University;
Scientific and Clinical Center of Toxicology named after Academician S.N. Golikov of the Federal Medical and Biological Agency

ORCID: 0009-0001-6759-526X

Balabanova O.L.

St. Petersburg State Chemical and Pharmaceutical University;
St. Petersburg Research Institute of Emergency Medicine named after. I.I. Dzhanelidze

ORCID: 0000-0002-8636-9858

Kuznetsova E.A.

Scientific and Clinical Center of Toxicology named after Academician S.N. Golikov of the Federal Medical and Biological Agency

ORCID: 0009-0008-7670-553X

Strelova O.Yu.

St. Petersburg State Chemical and Pharmaceutical University

ORCID: 0000-0001-6737-1023

Tursunov A.N.

Saint-Petersburg State University

ORCID: 0009-0007-3670-7576

Received:

16.03.2025

Accepted:

02.04.2025

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References:

Turkia M. Psycholytic dosing or ‘microdosing’ of Amanita muscaria (red fly agaric) mushrooms‒A retrospective case study. PsyArXiv. 2023. https://doi.org/10.31234/osf.io/dve46
Feeney K. On microdosing with the Mario mushroom. Journal of Psychedelic Studies. 2023;7(2):151-154. https://doi.org/10.1556/2054.2023.00303
Stebelska K. Fungal hallucinogens psilocin, ibotenic acid, and muscimol: analytical methods and biologic activities. Ther Drug Monit. 2013;35(4):420-442. https://doi.org/10.1097/FTD.0b013e31828741a5
Kim EJ, Kwon OS, Hur CG, Nyiramana MM, Lee DK, Hong SG, Han J, Kang D. Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus. Int J Mol Sci. 2021;22(17):9320. https://doi.org/10.3390/ijms22179320
Michelot D, Melendez-Howell L. M. Amanita muscaria: chemistry, biology, toxicology, and ethnomycology. Mycol Res. 2003;107(2):131-146. https://doi.org/10.1017/s0953756203007305
Satora L, Pach D, Butryn B, Hydzik P, Balicka-Slusarczyk B. Fly agaric (Amanita muscaria) poisoning, case report and review. Toxicon. 2005;45(7):941-943. https://doi.org/10.1016/j.toxicon.2005.01.005
Vendramin A, Brvar M. Amanita muscaria and Amanita pantherina poisoning: two syndromes. Toxicon. 2014;90:269-272. https://doi.org/10.1016/j.toxicon.2014.08.067
Mikaszewska-Sokolewicz MA, Pankowska S, Janiak M, Pruszczyk P, Łazowski T, Jankowski K. Coma in the course of severe poisoning after consumption of red fly agaric (Amanita muscaria). Acta Biochim Pol. 2016;63(1):181-182. https://doi.org/10.18388/abp.2015_1170
Order of the Ministry of Health and International Development of the Russian Federation of January 27, 2006 No. 40 «On the organization of conducting chemical-toxicological studies in the analytical diagnostics of adding alcohol, narcotic drugs, psychotropic and other toxic substances to the conditions of maintenance in the human body». (In Russ.).
Order of the Ministry of Health of Russia dated 18.12.2015 №933n (as amended on 25.03.2019) «On the procedure for conducting a medical examination for intoxication (alcohol, drugs or other toxic)» (In Russ.).
Clinical guidelines «Toxic effects of halogenated aliphatic and aromatic hydrocarbons» (approved by the Russian Ministry of Health) (In Russ.).
Clinical guidelines «Poisoning with benzodiazepines» (approved by the Ministry of Health of Russia) (In Russ.).
Ginterová P, Sokolová B, Ondra P, Znaleziona J, Petr J, Ševčík J, Maier V. Determination of mushroom toxins ibotenic acid, muscimol and muscarine by capillary electrophoresis coupled with electrospray tandem mass spectrometry. Talanta. 2014;125:242-247. https://doi.org/10.1016/j.talanta.2014.03.019
Stříbrný J, Sokol M, Merová B, Ondra P. GC/MS determination of ibotenic acid and muscimol in the urine of patients intoxicated with Amanita pantherina. Int J Legal Med. 2012;126(4):519-524. https://doi.org/10.1007/s00414-011-0599-9
Xu XM, Zhang JS, Huang BF, Han JL, Chen Q. Determination of ibotenic acid and muscimol in plasma by liquid chromatography-triple quadrupole mass spectrometry with bimolecular dansylation. J Chromatogr B Analyt Technol. Biomed Life Sci. 2020;1146:122-128. https://doi.org/10.1016/j.jchromb.2020.122128
State Pharmacopoeia of the Russian Federation XV edition. (In Russ.).
Scientific Working Group for Forensic Toxicology. Scientific Working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. J Anal Toxicol. 2013;37(7):452-474. https://doi.org/10.1093/jat/bkt054

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