Objective: to study of the pattern of vaginal microbiocenosis in girls with synechiae. Subjects and methods. Vaginal microbiocenosis was investigated in 26 girls aged 6 months to 8 years in the hormonal rest (neutral) period. Group 1 consisted of girls with external genital synechiae and none complaints, as well as unchanged vaginal mucosal color, and physiological discharge from the genital tract. Group 2 included girls with synechiae and complaints about burning sensations, discomfort concurrent with abnormal vaginal discharge. A control group (Group 3) comprised healthy girls undergoing a preventive examination. All the girls with vulvar synechiae received medical therapy involving the topical application of estrogen cream containing 1.0 mg of estriol. Polygynax virgo was used as a combination anti-inflammatory agent in a group of girls with signs of a vulvar inflammatory process. Results. Examination of the quantitative parameters of microbiocenosis in the examined groups of girls revealed the differences suggesting that there was a preponderance of dysbiotic processes in the girls with synechiae. Comparative analysis of complaints and visual signs showed that the baseline sum of clinical, pathological markers was higher in Group 2 girls with an inflammatory process whereas there was no significant group difference in the number of complaints and visual signs after antibacterial therapy with polygynax virgo. Conclusion. The given results suggest that the adhesive process of the external genitals in the girls was significantly more frequently accompanied by a preponderance of the anaerobic flora and by a tendency for aerobic microorganisms (Streptococcus spp., Enterobacteriaceae) to prevail, which may be regarded as a dysbiotic state. The antibacterial agent polygynax virgo as a therapeutic emulsion showed a high clinical efficacy in reducing the number of complaints and visual pathological signs of a vulvar inflammatory process.