One of the most important factors determining the quality of medical care is the effectiveness of healthcare workers’ activity, while it is significantly reduced when the employees develop professional burnout (PB) syndrome. Thus, the high level of PB in healthcare workers has been proven to cause more medical errors and the outflow of health professionals from the system. At the same time, the measures to level this syndrome, which are based on the understanding of the mechanisms of its development in the population, show the opposite effect. Such activities are currently not possible, since studies on this matter have been conducted in Russia either without the international validated instruments or with small samples. Objective — to study a regional model of PB in a large sample of healthcare workers. Material and methods. The study covered healthcare workers from 76 medical organizations of the Tomsk Region, who had filled out the questionnaire during the period from October 2015 to September 2016, which included the Maslach Burnout Inventory (MBI) to measure the level of PB, as well as the questions on the characteristics allowing qualification, work intensity and environment to be assessed. The study enrolled 4155 healthcare providers, including 1669 physicians and 2486 mid-level medical workers. There were 3128 (75.3%) and 1027 (24.7%) urban and rural healthcare providers, respectively. Results. Working more intensively (p <0.05), the rural physicians were found to be more susceptible to PB (p<0.05). The rural mid-level medical workers showed less labor intensity and a lower level of PB than their urban colleagues. In addition, some gender differences were found in labor intensity and PB levels. Conclusion. This study makes it possible to build a PB model in the Tomsk Region, which demonstrates substantial differences from the models shown in foreign studies.