A positive result of the ART program was first reported in the clinical case when a healthy child was born to a father having simultaneously two infertility factors difficult to overcome: non-obstructive azoospermia and reciprocal translocation. During the IVF program the following methods were used: microdissection testicular sperm extraction, a new method of single spermatozoa isolation from testicular tissue by differential centrifugation, a new embryo biopsy method at the compact morula stage, and high-throughput sequencing for preimplantation genetic testing for structural chromosomal abnormalities in the embryo.