Introduction. High frequency of cryo-thawed embryo transfer cycles in accordance to improvement of cryopreservation technologies, wide usage of GnRH antagonist for final oocyte maturation, «freeze all» strategy, preimplantational genetic testing has done evaluation of different schemes of endometrium preparation more actual. Objective. The aim of this study was evaluation of efficacy of endometrium preparation for frozen embryo transfer using hormonal replacement treatment (HRT) and HRT together with gonadotropin-releasing hormone antagonist (GnRH ant) to reveal the most appropriate scheme. Materials and methods. We conducted retrospective analysis of efficacy of endometrium preparation in cry-thawed embryo transfer cycles while using HRT and HRT together with GnRH antagonist. 230 patients suffering from infertility at the age between 22 and 39 years old were included into the study. 150 embryo transfers were performed in the group A (HRT) and 80 embryo transfers were performed in the group B (HRT with GnRH antagonist). The HRT scheme was the same in both study groups: estrogens in peroral and transdermal forms from 2—3rd day of cycle, progesterone treatment 6 days before blastocyst embryo transfer. Patients in the group B were treated with GnRH antagonist ganirelix 250 µg together with estrogens for 7 days. The primary outcome was clinical pregnancy rate (it was determined as gestational sac 21 day after embryo transfer). Endometrial thickness and dose of estrogens (daily and total) at the day of progesterone prescription were the secondary outcomes of our study. Conclusions. Results of the study did not show advantages of endometrium preparation for frozen embryo transfer using HRT together with GnRH antagonist at least within younger women in clinical pregnancy rate. Meanwhile further prospective trial are needed to reveal optimal scheme of endometrium preparation.