Objective — identification of biomarkers of early non-invasive diagnosis of uterine fibroids based on the study of lipid composition of blood plasma by mass spectrometry. Material and methods. The study involved 35 patients diagnosed with uterine fibroids (UF), confirmed intraoperatively and finally verified by histological examination, and 15 patients who made up the comparison group (patients with infertility, intrauterine septum). All 50 patients underwent reconstructive surgery with laparoscopic access in the Department of Operative Gynecology of the National Medical Reseach Center for Obstetrics, Gynecology and Perinatology named after Acad. V.I .Kulakov. According to the examination program, prior to surgery, patients were sampled for determination of lipid composition. Qualitative and quantitative assessment of lipidoma was carried out using electrospray mass spectrometry. The data obtained were analyzed using the multivariate method PLS-DA. The created statistical models revealed lipids, potential biomarkers of UF. Results. Metabolic profiling of blood plasma was performed in patients with UF. In plasma samples, 267 lipid compounds were identified. Phosphotidylcholines and sphingomyelins were identified, which subsequently can be considered as potential biomarkers of the disease. Conclusion. Significant differences in the lipid composition of blood plasma in patients with UF were identified for the first time. Further research is needed to assess the feasibility of using these biomarkers in early diagnosis of the disease and predicting the recurrence of the disease.